Anti-Mycobacterium tuberculosis antibody (FITC) (ab20962)


  • Product nameAnti-Mycobacterium tuberculosis antibody (FITC)
    See all Mycobacterium tuberculosis primary antibodies
  • Description
    Rabbit polyclonal to Mycobacterium tuberculosis (FITC)
  • ConjugationFITC. Ex: 493nm, Em: 528nm
  • SpecificityMinimum of 2 major M. tuberculosis bands by immunoelectrophoresis (gamma & beta). This antiserum has not been adsorbed and may react with related microorganisms.
  • Tested applicationsSuitable for: IHC-P, ICC/IFmore details
  • Species reactivity
  • Immunogen

    Purified PPD.

  • General notes

    The antibody is covalently coupled with high purity Isomer I of fluorescein isothiocyanate. Care is taken to ensure complete removal of any free fluorescein from the final product.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: 10mg/ml BSA, 0.01M PBS, pH 7.2
  • Concentration information loading...
  • PurityIgG fraction
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab20962 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/10 - 1/50.
ICC/IF Use at an assay dependent dilution. Acetone fixation is recommended.


  • RelevanceMycobacterium tuberculosis is the most common cause of tuberculosis. Primary infection begins with inhalation of 1 to 10 aerosolised bacilli. The pathogenicity of the organism is determined by its ability to escape host immune responses as well as eliciting delayed hypersensitivity. Alveolar macrophages engulf the invading cells but are unable to mount an effective defense. Several virulence factors are responsible for this apparent failure; most notably in the mycobacterial cell wall are the cord factor, lipoarabinomannan, and the 65 kd heat shock protein or HSP65. The emergence of new strains of resistant Mycobacterium tuberculosis has created new interest in clinical diagnosis. Studies have shown immunohistochemical techniques to be superior to conventional special stains. Thus the demonstration of mycobacterial antigens are not only useful in establishing mycobacterial aetiology, but can also be used as an alternative method to the conventional Ziehl-Neelsen method.
  • Alternative names
    • M. tuberculosis antibody

References for Anti-Mycobacterium tuberculosis antibody (FITC) (ab20962)

This product has been referenced in:
  • Bouttier M  et al. Alu repeats as transcriptional regulatory platforms in macrophage responses to M. tuberculosis infection. Nucleic Acids Res N/A:N/A (2016). Human . Read more (PubMed: 27604870) »
  • Verway M  et al. Vitamin D induces interleukin-1ß expression: paracrine macrophage epithelial signaling controls M. tuberculosis infection. PLoS Pathog 9:e1003407 (2013). ICC/IF ; Mouse . Read more (PubMed: 23762029) »

See all 3 Publications for this product

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Thank you for your enquiry.

I am sorry to confirm that after further investigation with our source, I can confirm that ab20962 has not specifically been tested for detection of BCG.

If they would like totryit for detection of BCG,...

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Thank you for your enquiry. I regret to inform you that the originator of this antibody no longer has sufficient stocks for us to be able to make this product available to our customers. We have therefore been forced to withdraw ab34957 from our pr...

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Thank you for your enquiry. I can confirm that PPD (purified protein derivative) immunogen for this Mycobacterium tuberculosis antibody is a commercially available preparation used in skin testing for tuberculosis. PPD tuberculin is a precipitate o...

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