Anti-Mycobacterium tuberculosis antibody (HRP) (ab21189)

Overview

  • Product nameAnti-Mycobacterium tuberculosis antibody (HRP)
    See all Mycobacterium tuberculosis primary antibodies
  • Description
    Rabbit polyclonal to Mycobacterium tuberculosis (HRP)
  • ConjugationHRP
  • SpecificityThis antiserum has not been absorbed and may react with related species. Reactive with other Mycobacteria species including M. avium, M. phlei and M. parafortuitum. Antibody is non-reactive with E. coli K12, Salmonella typhimurium, Pseudomonas aeruginosa, Streptococcus (group B), Candida albicans and Neisseria meningitidis.
  • Tested applicationsSuitable for: IHC-Pmore details
  • Immunogen

    Tissue/ cell preparation.Purified PPD

  • General notes

    IgG fraction covalently coupled to a highly purified preparation of horseradish peroxidase (RZ>3). Care is taken to ensure adequate conjugation while reserving maximum enzyme activity. Free enzyme is absent. Estimated Molar HRP: IgG substitution is 2-3

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 0.002% Thimerosal (merthiolate)
    Constituents: PBS, 10mg/ml BSA
  • Concentration information loading...
  • PurityIgG fraction
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab21189 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent dilution.

Target

  • RelevanceMycobacterium tuberculosis is the most common cause of tuberculosis. Primary infection begins with inhalation of 1 to 10 aerosolised bacilli. The pathogenicity of the organism is determined by its ability to escape host immune responses as well as eliciting delayed hypersensitivity. Alveolar macrophages engulf the invading cells but are unable to mount an effective defense. Several virulence factors are responsible for this apparent failure; most notably in the mycobacterial cell wall are the cord factor, lipoarabinomannan, and the 65 kd heat shock protein or HSP65. The emergence of new strains of resistant Mycobacterium tuberculosis has created new interest in clinical diagnosis. Studies have shown immunohistochemical techniques to be superior to conventional special stains. Thus the demonstration of mycobacterial antigens are not only useful in establishing mycobacterial aetiology, but can also be used as an alternative method to the conventional Ziehl-Neelsen method.
  • Alternative names
    • M. tuberculosis antibody

References for Anti-Mycobacterium tuberculosis antibody (HRP) (ab21189)

ab21189 has not yet been referenced specifically in any publications.

Product Wall

As a follow up to my previous message, I can now confirm that ab21189 does not require antigen retrieval. I hope this information helps but please don't hesitate to contact me should you require any additional assistance.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"