Anti-Mycobacterium tuberculosis (Hsp-65) antibody [BDI577] (ab20519)

Overview

  • Product name
    Anti-Mycobacterium tuberculosis (Hsp-65) antibody [BDI577]
    See all Mycobacterium tuberculosis (Hsp-65) primary antibodies
  • Description
    Mouse monoclonal [BDI577] to Mycobacterium tuberculosis (Hsp-65)
  • Specificity
    Reacts with HSP65 (grOEL) of M. tuberculosis. Does not react with M. bovis, M. avium, M. phlei, M. parafortuitum, Rhodococcus species, B. subtilis, S. pneumoniae or E. coli.
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with Mycobacterium tuberculosis. Not yet tested in other species.
  • Immunogen

    Purified protein derivative

  • General notes


    This antibody reacts with Mycobacterium tuberculosis HSP65

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.1% Sodium Azide
    Constituents: 0.01M PBS, pH 7.2
  • Concentration information loading...
  • Purity
    Protein A purified
  • Primary antibody notes
    This antibody reacts with Mycobacterium tuberculosis HSP65
  • Clonality
    Monoclonal
  • Clone number
    BDI577
  • Isotype
    IgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab20519 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/20 - 1/200. Predicted molecular weight: 60 kDa.
ELISA 1/10 - 1/50.

Target

  • Relevance
    Mycobacterium tuberculosis is the most common cause of tuberculosis. Primary infection begins with inhalation of 1 to 10 aerosolised bacilli. The pathogenicity of the organism is determined by its ability to escape host immune responses as well as eliciting delayed hypersensitivity. Alveolar macrophages engulf the invading cells but are unable to mount an effective defense. Several virulence factors are responsible for this apparent failure; most notably in the mycobacterial cell wall are the cord factor, lipoarabinomannan, and the 65 kd heat shock protein or HSP65. The emergence of new strains of resistant Mycobacterium tuberculosis has created new interest in clinical diagnosis. Studies have shown immunohistochemical techniques to be superior to conventional special stains. Thus the demonstration of mycobacterial antigens are not only useful in establishing mycobacterial aetiology, but can also be used as an alternative method to the conventional Ziehl-Neelsen method.
  • Cellular localization
    Cytoplasmic
  • Alternative names
    • 60 kDa chaperonin 2 antibody
    • 65 kDa antigen antibody
    • Antigen A antibody
    • Cell wall protein A antibody
    • groEL antibody
    • GroEL protein 2 antibody
    • GroEL2 antibody
    • Heat shock protein 65 antibody
    • Hsp65 antibody
    see all

Anti-Mycobacterium tuberculosis (Hsp-65) antibody [BDI577] images

  • Anti-Mycobacterium tuberculosis (Hsp-65) antibody [BDI577] (ab20519) at 1/200 dilution + Mycobacterium tuberculosis H37Rv whole cell lysate

    Secondary
    Goat anti-mouse antibody conjugated to HRP at 1/2000 dilution

    Predicted band size : 60 kDa

    This image is courtesy of an Abreview submitted by Dr Lay Harn Gam

    See Abreview

References for Anti-Mycobacterium tuberculosis (Hsp-65) antibody [BDI577] (ab20519)

This product has been referenced in:
  • Seeliger JC  et al. A riboswitch-based inducible gene expression system for mycobacteria. PLoS One 7:e29266 (2012). WB . Read more (PubMed: 22279533) »
  • Seeliger JC  et al. Elucidation and Chemical Modulation of Sulfolipid-1 Biosynthesis in Mycobacterium tuberculosis. J Biol Chem 287:7990-8000 (2012). WB . Read more (PubMed: 22194604) »

See all 2 Publications for this product

Product Wall

Vielen Dank für Ihre Anfrage. Da die Epitope der beiden Klone wie gesagt leider nicht kartiert wurden, kann nicht mit Sicherheit gesagt werden, ob es sich um unterschiedliche Epitope handelt; die Wahrscheinlichkeit dafür ist jedoch durchaus gegeben....

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Vielen Dank für Ihre Anfrage. Bei ab20519 und ab20045 handelt es sich um zwei verschiedene Klone, die bei der Herstellung von monoklonalen Antikörpern gegen das Hsp65 von Mycobacterium tuberculosis entstanden sind, nämlich [BDI577] und [BDI578]. Die...

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Vielen Dank für Ihre Anfrage. In der Tat haben wir einige polyklonalen HSP60- Antikörper in unserem Katalog verfügbar, unter anderem zum Beispiel ab105853: ein Kaninchen-polyklonaler, der gegen das humane Volllängenprotein gerichtet ist und für West...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mycobacterium tuberculosis Cell lysate - whole cell (Mycobacterium tuberculosis H37Rv)
Loading amount
40 µg
Specification
Mycobacterium tuberculosis H37Rv
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Dr. Lay Harn Gam

Verified customer

Submitted Mar 16 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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