Anti-Mycobacterium tuberculosis (Hsp-65) antibody [BDI577] (ab20519)


  • Product name
    Anti-Mycobacterium tuberculosis (Hsp-65) antibody [BDI577]
  • Description
    Mouse monoclonal [BDI577] to Mycobacterium tuberculosis (Hsp-65)
  • Host species
  • Specificity
    Reacts with HSP65 (grOEL) of M. tuberculosis. Does not react with M. bovis, M. avium, M. phlei, M. parafortuitum, Rhodococcus species, B. subtilis, S. pneumoniae or E. coli.
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with Mycobacterium tuberculosis. Not yet tested in other species.
  • Immunogen

    Purified protein derivative

  • General notes

    This antibody reacts with Mycobacterium tuberculosis HSP65

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. 


  • Form
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.1% Sodium Azide
    Constituents: 0.01M PBS, pH 7.2
  • Concentration information loading...
  • Purity
    Protein A purified
  • Primary antibody notes
    This antibody reacts with Mycobacterium tuberculosis HSP65
  • Clonality
  • Clone number
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab20519 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/20 - 1/200. Predicted molecular weight: 60 kDa.
ELISA 1/10 - 1/50.


  • Relevance
    Mycobacterium tuberculosis is the most common cause of tuberculosis. Primary infection begins with inhalation of 1 to 10 aerosolised bacilli. The pathogenicity of the organism is determined by its ability to escape host immune responses as well as eliciting delayed hypersensitivity. Alveolar macrophages engulf the invading cells but are unable to mount an effective defense. Several virulence factors are responsible for this apparent failure; most notably in the mycobacterial cell wall are the cord factor, lipoarabinomannan, and the 65 kd heat shock protein or HSP65. The emergence of new strains of resistant Mycobacterium tuberculosis has created new interest in clinical diagnosis. Studies have shown immunohistochemical techniques to be superior to conventional special stains. Thus the demonstration of mycobacterial antigens are not only useful in establishing mycobacterial aetiology, but can also be used as an alternative method to the conventional Ziehl-Neelsen method.
  • Cellular localization
  • Alternative names
    • 60 kDa chaperonin 2 antibody
    • 65 kDa antigen antibody
    • Antigen A antibody
    • Cell wall protein A antibody
    • groEL antibody
    • GroEL protein 2 antibody
    • GroEL2 antibody
    • Heat shock protein 65 antibody
    • Hsp65 antibody
    see all


  • Anti-Mycobacterium tuberculosis (Hsp-65) antibody [BDI577] (ab20519) at 1/200 dilution + Mycobacterium tuberculosis H37Rv whole cell lysate

    Goat anti-mouse antibody conjugated to HRP at 1/2000 dilution

    Predicted band size: 60 kDa

    Please refer to abreview for further experimental details.

    See Abreview


This product has been referenced in:
  • Touchette MH  et al. A Screen for Protein-Protein Interactions in Live Mycobacteria Reveals a Functional Link between the Virulence-Associated Lipid Transporter LprG and the Mycolyltransferase Antigen 85A. ACS Infect Dis 3:336-348 (2017). Read more (PubMed: 28276676) »
  • Seeliger JC  et al. A riboswitch-based inducible gene expression system for mycobacteria. PLoS One 7:e29266 (2012). WB . Read more (PubMed: 22279533) »

See all 4 Publications for this product

Customer reviews and Q&As

Vielen Dank für Ihre Anfrage. Da die Epitope der beiden Klone wie gesagt leider nicht kartiert wurden, kann nicht mit Sicherheit gesagt werden, ob es sich um unterschiedliche Epitope handelt; die Wahrscheinlichkeit dafür ist jedoch durchaus gegeben....

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Vielen Dank für Ihre Anfrage. Bei ab20519 und ab20045 handelt es sich um zwei verschiedene Klone, die bei der Herstellung von monoklonalen Antikörpern gegen das Hsp65 von Mycobacterium tuberculosis entstanden sind, nämlich [BDI577] und [BDI578]. Die...

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Vielen Dank für Ihre Anfrage. In der Tat haben wir einige polyklonalen HSP60- Antikörper in unserem Katalog verfügbar, unter anderem zum Beispiel ab105853: ein Kaninchen-polyklonaler, der gegen das humane Volllängenprotein gerichtet ist und für West...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Western blot
Mycobacterium tuberculosis Cell lysate - whole cell (Mycobacterium tuberculosis H37Rv)
Loading amount
40 µg
Mycobacterium tuberculosis H37Rv
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C

Dr. Lay Harn Gam

Verified customer

Submitted Mar 16 2009


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