Overview

  • Product nameAnti-MyD88 antibody [1B4]
    See all MyD88 primary antibodies
  • Description
    Mouse monoclonal [1B4] to MyD88
  • Tested applicationsSuitable for: WB, Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length Human MyD88 produced in HEK293T cells (NP_002459).

  • Positive control
    • HEK293T cell lysate transfected with pCMV6-ENTRY MyD88 cDNA; HEK293T cells transfected with pCMV6-ENTRY MyD88 overexpress plasmid; COS7 cells transiently transfected by pCMV6-ENTRY MyD88; HeLa and Jurkat cells.
  • General notesDilute in PBS (pH7.3) before use. Stable for 12 months from date of receipt.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab119048 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Predicted molecular weight: 33 kDa.
Flow Cyt 1/100. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
ICC/IF 1/100.

Target

  • FunctionAdapter protein involved in the Toll-like receptor and IL-1 receptor signaling pathway in the innate immune response. Acts via IRAK1, IRAK2, IRF7 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Increases IL-8 transcription. Involved in IL-18-mediated signaling pathway.
  • Tissue specificityUbiquitous.
  • Involvement in diseaseDefects in MYD88 are the cause of MYD88 deficiency (MYD88D) [MIM:612260]; also known as recurrent pyogenic bacterial infections due to MYD88 deficiency. Patients suffer from autosomal recessive, life-threatening, often recurrent pyogenic bacterial infections, including invasive pneumococcal disease, and die between 1 and 11 months of age. Surviving patients are otherwise healthy, with normal resistance to other microbes, and their clinical status improved with age.
  • Sequence similaritiesContains 1 death domain.
    Contains 1 TIR domain.
  • DomainThe intermediate domain (ID) is required for the phosphorylation and activation of IRAK.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • Mutant myeloid differentiation primary response 88 antibody
    • MYD 88 antibody
    • Myd88 antibody
    • MYD88_HUMAN antibody
    • MYD88D antibody
    • Myeloid differentiation marker 88 antibody
    • Myeloid differentiation primary response 88 antibody
    • Myeloid differentiation primary response gene (88) antibody
    • Myeloid differentiation primary response gene 88 antibody
    • Myeloid differentiation primary response gene antibody
    • Myeloid differentiation primary response protein MyD88 antibody
    • OTTHUMP00000161718 antibody
    • OTTHUMP00000208595 antibody
    • OTTHUMP00000209058 antibody
    • OTTHUMP00000209059 antibody
    • OTTHUMP00000209060 antibody
    see all

Anti-MyD88 antibody [1B4] images



  • Predicted band size : 33 kDa

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: MyD88 knockout HAP1 cell lysate (20 µg)
    Lane 3: Jurkat cell lysate (20 µg)
    Lane 4: Molt-4 cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab119048 observed at 37 kDa. Red - loading control, ab176560, observed at 52 kDa.

    ab119048 was shown to specifically react with MyD88 when MyD88 knockout samples were used. Wild-type and MyD88 knockout samples were subjected to SDS-PAGE.  Ab119048 and ab176560 (loading control to alpha Tubulin) were diluted at 1/2000 and 1/10,000 dilution respectively and incubated overnight at 4C. Blots were developed with IRDye® 800CW Goat anti-Mouse IgG (H + L) and IRDye® 680 Goat anti-Rabbit IgG (H + L) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

  • All lanes : Anti-MyD88 antibody [1B4] (ab119048) at 1/2000 dilution

    Lane 1 : HEK293T lysate transfected with pCMV6-ENTRY control cDNA
    Lane 2 : HEK293T lysate transfected with pCMV6-ENTRY MyD88 cDNA

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 33 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC202253)
  • ab119048 at 1/100 dilution staining MyD88 in COS7 cells transiently transfected with pCMV6-ENTRY MyD88 by Immunofluorescence.
  • ab119048 at 1/100 dilution, staining MyD88 in HEK293T cells transfected with pCMV6-ENTRY MyD88 overexpress plasmid(Red), or an empty vector control plasmid (Blue) by Flow Cytometry.
  • ab119048 at 1/100 dilution staining MyD88 in HeLa cells by Flow cytometry (Red) compared to a nonspecific negative control antibody (Blue).
  • ab119048 at 1/100 dilution staining MyD88 in Jurkat cells by Flow cytometry (Red) compared to a nonspecific negative control antibody (Blue).

References for Anti-MyD88 antibody [1B4] (ab119048)

ab119048 has not yet been referenced specifically in any publications.

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