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Synthetic peptide corresponding to Human MyD88 (internal sequence). ab2068 antibody was raised against a 16 amino acid peptide near the center of human MD88
(Peptide available as
Our Abpromise guarantee covers the use of ab2068 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 5 µg/ml.|
|IHC-P||Use a concentration of 2 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 35 kDa.Can be blocked with Human MyD88 peptide (ab7882).
Use a modified RIPA lysis buffer: 150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Remove cell debris by centrifugation. Boil the lysate for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% ß-mercaptoethanol). Block the membrane with 5% non-fat dry milk in TBS overnight at 4°C or 2 hours at room temperature.
|Flow Cyt||Use at an assay dependent concentration.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
ab2068 staining MyD88 in Human sigma / diverticulitis tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde; antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/50) for 1 hour at 25°C. An undiluted Biotin-conjugated Goat anti-rabbit polyclonal was used as the secondary antibody
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