The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 4 - 6 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa).
Use a concentration of 5 - 10 µg/ml. Recombinant Antigen captured by rabbit antibody and ab28763 used as reporter
Adapter protein involved in the Toll-like receptor and IL-1 receptor signaling pathway in the innate immune response. Acts via IRAK1, IRAK2, IRF7 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Increases IL-8 transcription. Involved in IL-18-mediated signaling pathway.
Involvement in disease
Defects in MYD88 are the cause of MYD88 deficiency (MYD88D) [MIM:612260]; also known as recurrent pyogenic bacterial infections due to MYD88 deficiency. Patients suffer from autosomal recessive, life-threatening, often recurrent pyogenic bacterial infections, including invasive pneumococcal disease, and die between 1 and 11 months of age. Surviving patients are otherwise healthy, with normal resistance to other microbes, and their clinical status improved with age.
Contains 1 death domain. Contains 1 TIR domain.
The intermediate domain (ID) is required for the phosphorylation and activation of IRAK.
ab28763 (4µg/ml) staining of paraffin embedded Human Tonsil shows staining of clusters of cells some of which are likely plasma cells. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.
Liu D et al. MyD88 expression in the rat dental follicle: implications for osteoclastogenesis and tooth eruption. Eur J Oral Sci118:333-41 (2010).
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Szajnik M et al. TLR4 signaling induced by lipopolysaccharide or paclitaxel regulates tumor survival and chemoresistance in ovarian cancer. Oncogene28:4353-63 (2009).
Read more (PubMed: 19826413) »