Full length protein (Cow).
Our Abpromise guarantee covers the use of ab7349 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-FoFr||Use at an assay dependent concentration. PubMed: 18678887|
|IHC-P||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration.|
|ELISA||Use at an assay dependent concentration.|
|RIA||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration. PubMed: 23584610|
ab7349 staining Myelin Basic Protein in human trigeminal ganglia tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 10% serum for 1 hour at 20°C; antigen retrieval was by heat mediation in citrate buffer, pH 6.0. Samples were incubated with primary antibody (1/1000 in 10% NDS) for 16 hours at 22°C. ab150165, a goat anti-rat IgG H&L (Alexa Fluor® 488) preadsorbed polyclonal (1/1000) was used as the secondary antibody.