Anti-Myelin Basic Protein antibody [12] (ab7349)

Overview

  • Product nameAnti-Myelin Basic Protein antibody [12]
    See all Myelin Basic Protein primary antibodies
  • Description
    Rat monoclonal [12] to Myelin Basic Protein
  • SpecificityThe antibody reacts weakly with peptides ending in the Phe 91 where the 91-92 Phe-Phe bond is broken. Synthetic peptide 82-99 reacts very well, as does intact MBP. Further epitope analysis indicates binding to a region defined by amino acids 82-87 (DENPVV). Mapped by Geyson method to DENPW method.
  • Tested applicationsIHC-FoFr, IHC-P, WB, ELISA, RIA, IHC-Fr, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Sheep, Rabbit, Guinea pig, Cow, Dog, Human, Pig, Apteronotus leptorhynchus
  • Immunogen

    Full length protein (Cow).

  • EpitopeAmino acids 82-87 (DENPVV).

Properties

Applications

Our Abpromise guarantee covers the use of ab7349 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr Use at an assay dependent concentration. PubMed: 18678887
IHC-P Use at an assay dependent concentration.
WB Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
RIA Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration. PubMed: 23584610

Target

  • FunctionThe classic group of MBP isoforms (isoform 4-isoform 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non-classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T-cells and neural cells. Differential splicing events combined with optional post-translational modifications give a wide spectrum of isomers, with each of them potentially having a specialized function. Induces T-cell proliferation.
  • Tissue specificityMBP isoforms are found in both the central and the peripheral nervous system, whereas Golli-MBP isoforms are expressed in fetal thymus, spleen and spinal cord, as well as in cell lines derived from the immune system.
  • Involvement in diseaseNote=The reduction in the surface charge of citrullinated and/or methylated MBP could result in a weakened attachment to the myelin membrane. This mechanism could be operative in demyelinating diseases such as chronical multiple sclerosis (MS), and fulminating MS (Marburg disease).
  • Sequence similaritiesBelongs to the myelin basic protein family.
  • Developmental stageExpression begins abruptly in 14-16 week old fetuses. Even smaller isoforms seem to be produced during embryogenesis; some of these persisting in the adult. Isoform 4 expression is more evident at 16 weeks and its relative proportion declines thereafter.
  • Post-translational
    modifications
    Several charge isomers of MBP; C1 (the most cationic, least modified, and most abundant form), C2, C3, C4, C5, C6, C7, C8-A and C8-B (the least cationic form); are produced as a result of optional PTM, such as phosphorylation, deamidation of glutamine or asparagine, arginine citrullination and methylation. C8-A and C8-B contain each two mass isoforms termed C8-A(H), C8-A(L), C8-B(H) and C8-B(L), (H) standing for higher and (L) for lower molecular weight. C3, C4 and C5 are phosphorylated. The ratio of methylated arginine residues decreases during aging, making the protein more cationic.
    The N-terminal alanine is acetylated (isoform 3, isoform 4, isoform 5 and isoform 6).
    Arg-241 was found to be 6% monomethylated and 60% symmetrically dimethylated.
  • Cellular localizationMyelin membrane. Cytoplasmic side of myelin.
  • Information by UniProt
  • Database links
  • Alternative names
    • GDB antibody
    • Golli MBP antibody
    • Golli MBP; myelin basic protein antibody
    • Hemopoietic MBP antibody
    • HMBPR antibody
    • HUGO antibody
    • MBP antibody
    • MBP_HUMAN antibody
    • MGC99675 antibody
    • MLD antibody
    • Myelin A1 Protein antibody
    • Myelin basic protein antibody
    • Myelin Deficient antibody
    • Myelin Membrane Encephalitogenic Protein antibody
    • OTTHUMP00000163776 antibody
    • OTTHUMP00000174387 antibody
    • OTTHUMP00000174388 antibody
    • SHI antibody
    • Shiverer antibody
    • SP antibody
    see all

Anti-Myelin Basic Protein antibody [12] images

  • ab7349 at 1/100 staining mouse spinal cord tissue sections by IHC-Fr. The tissue was formaldehyde fixed and blocked with 3% serum prior to incubation with the antibody for 18 hours. A biotinylated horse anti-rat antibody was used as the secondary.

    See Abreview

  • ab7349 staining Myelin Basic Protein in murine cerebral cortex tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was not fixed. Samples were blocked with 2.5% serum for 1 hour followed by incubation with the primary antibody at a 1/200 dilution for 18 hours. A Biotin-conjugated rabbit anti-rat polyclonal was used as the secondary antibody at a 1/500 dilution.
  • Anti-Myelin Basic Protein antibody [12] (ab7349) + Mouse brain tissue lysate

    Observed band size : 19,26 kDa (why is the actual band size different from the predicted?)

References for Anti-Myelin Basic Protein antibody [12] (ab7349)

This product has been referenced in:
  • Rodriguez-Grande B  et al. The acute-phase protein PTX3 is an essential mediator of glial scar formation and resolution of brain edema after ischemic injury. J Cereb Blood Flow Metab 34:480-8 (2014). Rat . Read more (PubMed: 24346689) »
  • Lehnert S  et al. Action potential generation in an anatomically constrained model of medial superior olive axons. J Neurosci 34:5370-84 (2014). Read more (PubMed: 24719114) »

See all 31 Publications for this product

Product Wall

Application Immunohistochemistry (Frozen sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: Rm°C
Sample Human Tissue sections (Human Myelin/Oligodendrocytes in Mouse Shiverer Br)
Specification Human Myelin/Oligodendrocytes in Mouse Shiverer Br
Permeabilization Yes - Triton
Fixative Paraformaldehyde
Username

Dr. Fraser Sim

Verified customer

Submitted Sep 16 2014

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Zebra finch Tissue sections (Brain)
Specification Brain
Fixative Paraformaldehyde
Antigen retrieval step None
Permeabilization Yes - Triton
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10%
Username

Mr. Adam Piraino

Verified customer

Submitted Mar 28 2013

Thank you for your reply.

The following antibodies are suitable for your primary antibody ab7349 which is a rat IgG2a:

Biotinylated:

ab99668

http://www.abcam.com/index.html?datasheet=99668 (or use the following: ...

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Before I can recommend a secondary antibody to you, I would need to know the following:

What application are you using, IHC-P or IHC-Fr?

Shall the antibody be conjugated with an enzyme or with a Fluorochrom?

Will you use the...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Apteronotus leptorhynchus Tissue lysate - whole (Brain)
Loading amount 50 µg
Specification Brain
Gel Running Conditions Reduced Denaturing (4-15%)
Blocking step Milk as blocking agent for 15 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

Submitted Jan 23 2013

Thank you for your enquiry.

I can confirm that the anti-Myelin Basic Protein antibody [12] - Oligodendrocyte Marker ab7349 is sold as tissue culture supernatant. Unpurified antibodies, such as those sold as whole antiserum, ascites or tissue c...

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Unfortunately there is no estimate for the purity level.
The use of unpurified antibody should not interefere with the experiements.

Thank you for your reply.

As indicated on the datasheet, ab7349 is sold as a tissue culture supernatant.
Unpurified antibodies, such as those sold as whole antiserum, ascites or tissue culture supernatant will not have a concentration stat...

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Thank you for contacting us.

The antibody that would be suitable for the detection of the human myelin basic protein is the clone [12]. The epitope of this clone has 100% homology with the sequence you provided.
The 2 Abcam references that...

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Thank you for contacting us and for reporting the problem you experienced with the antibody ab7349 lot GR105321-1.

I am sorry that the product you received did not perform as stated on the datasheet.
We have not received any other negative...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"