Anti-Myelin Basic Protein antibody [12] (ab92406)

Overview

  • Product name
    Anti-Myelin Basic Protein antibody [12]
    See all Myelin Basic Protein primary antibodies
  • Description
    Rat monoclonal [12] to Myelin Basic Protein
  • Specificity
    Myelin basic protein
  • Tested applications
    Suitable for: ELISA, IHC-Fr, RIAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Sheep, Rabbit, Chicken, Guinea pig, Cow, Human, Pig, Xenopus laevis
  • Immunogen

    Synthetic peptide (Cow)

  • Epitope
    ab92406 reacts weakly with peptides ending in the Phe 91 where the 91-92 Phe-Phe bond is broken. Synthetic peptide 82-99 reacts very well, as does intact MBP. Further epitope analysis indicates binding to a region defined by amino acids 82-87.
  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and as such, we will be discontinuing this product, which has been generated by the ascites method, within the next year. We are sorry for any inconvenience this may cause. If you would like help finding an alternative product, please do not hesitate to contact our scientific support team.

Properties

Applications

Our Abpromise guarantee covers the use of ab92406 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
IHC-Fr
RIA
  • Application notes
    ELISA: Use at an assay dependent dilution.
    IHC-Fr: Use at an assay dependent dilution.
    RIA: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      The classic group of MBP isoforms (isoform 4-isoform 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non-classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T-cells and neural cells. Differential splicing events combined with optional post-translational modifications give a wide spectrum of isomers, with each of them potentially having a specialized function. Induces T-cell proliferation.
    • Tissue specificity
      MBP isoforms are found in both the central and the peripheral nervous system, whereas Golli-MBP isoforms are expressed in fetal thymus, spleen and spinal cord, as well as in cell lines derived from the immune system.
    • Involvement in disease
      Note=The reduction in the surface charge of citrullinated and/or methylated MBP could result in a weakened attachment to the myelin membrane. This mechanism could be operative in demyelinating diseases such as chronical multiple sclerosis (MS), and fulminating MS (Marburg disease).
    • Sequence similarities
      Belongs to the myelin basic protein family.
    • Developmental stage
      Expression begins abruptly in 14-16 week old fetuses. Even smaller isoforms seem to be produced during embryogenesis; some of these persisting in the adult. Isoform 4 expression is more evident at 16 weeks and its relative proportion declines thereafter.
    • Post-translational
      modifications
      Several charge isomers of MBP; C1 (the most cationic, least modified, and most abundant form), C2, C3, C4, C5, C6, C7, C8-A and C8-B (the least cationic form); are produced as a result of optional PTM, such as phosphorylation, deamidation of glutamine or asparagine, arginine citrullination and methylation. C8-A and C8-B contain each two mass isoforms termed C8-A(H), C8-A(L), C8-B(H) and C8-B(L), (H) standing for higher and (L) for lower molecular weight. C3, C4 and C5 are phosphorylated. The ratio of methylated arginine residues decreases during aging, making the protein more cationic.
      The N-terminal alanine is acetylated (isoform 3, isoform 4, isoform 5 and isoform 6).
      Arg-241 was found to be 6% monomethylated and 60% symmetrically dimethylated.
    • Cellular localization
      Myelin membrane. Cytoplasmic side of myelin.
    • Information by UniProt
    • Database links
    • Alternative names
      • GDB antibody
      • Golli MBP antibody
      • Golli MBP; myelin basic protein antibody
      • Hemopoietic MBP antibody
      • HMBPR antibody
      • HUGO antibody
      • MBP antibody
      • MBP_CAVPO antibody
      • MBP_HUMAN antibody
      • MGC99675 antibody
      • MLD antibody
      • Myelin A1 protein antibody
      • Myelin A1 Protein, basic antibody
      • Myelin basic protein antibody
      • Myelin Deficient antibody
      • Myelin membrane encephalitogenic protein antibody
      • OTTHUMP00000163776 antibody
      • OTTHUMP00000174387 antibody
      • OTTHUMP00000174388 antibody
      • SHI antibody
      • Shiverer antibody
      • SP antibody
      see all

    References

    ab92406 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Thank you for contacting us.

    The antibody that would be suitable for the detection of the human myelin basic protein is the clone [12]. The epitope of this clone has 100% homology with the sequence you provided.
    The 2 Abcam references that...

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    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Xenopus laevis Tissue sections (optic nerve)
    Specification
    optic nerve
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.1% Triton-X
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted May 03 2012

    I am happy to provide these codes for participation in our 100% Abreview discount program. We normally will not issue these retroactively but are happy to make an exception in this case for you. Should you wish to test further products please contact o...

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