Anti-Myelin Basic Protein antibody [7G7] (ab78156)

Overview

  • Product nameAnti-Myelin Basic Protein antibody [7G7]
    See all Myelin Basic Protein primary antibodies
  • Description
    Mouse monoclonal [7G7] to Myelin Basic Protein
  • Specificityab78156 detects four transcripts (molecular weights of 21.5, 18.5, 17.2, 14 kDa).
  • Tested applicationsSuitable for: Flow Cyt, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Purified bovine myelin

  • Positive control
    • Crude rat spinal cord homogenate. Peripheral nerve homogenate. Rat mixed neuron/glial cultures.

Properties

Applications

Our Abpromise guarantee covers the use of ab78156 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/50. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
WB 1/10000. Predicted molecular weight: 33 kDa.
ICC/IF 1/1000.

Target

  • FunctionThe classic group of MBP isoforms (isoform 4-isoform 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non-classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T-cells and neural cells. Differential splicing events combined with optional post-translational modifications give a wide spectrum of isomers, with each of them potentially having a specialized function. Induces T-cell proliferation.
  • Tissue specificityMBP isoforms are found in both the central and the peripheral nervous system, whereas Golli-MBP isoforms are expressed in fetal thymus, spleen and spinal cord, as well as in cell lines derived from the immune system.
  • Involvement in diseaseNote=The reduction in the surface charge of citrullinated and/or methylated MBP could result in a weakened attachment to the myelin membrane. This mechanism could be operative in demyelinating diseases such as chronical multiple sclerosis (MS), and fulminating MS (Marburg disease).
  • Sequence similaritiesBelongs to the myelin basic protein family.
  • Developmental stageExpression begins abruptly in 14-16 week old fetuses. Even smaller isoforms seem to be produced during embryogenesis; some of these persisting in the adult. Isoform 4 expression is more evident at 16 weeks and its relative proportion declines thereafter.
  • Post-translational
    modifications
    Several charge isomers of MBP; C1 (the most cationic, least modified, and most abundant form), C2, C3, C4, C5, C6, C7, C8-A and C8-B (the least cationic form); are produced as a result of optional PTM, such as phosphorylation, deamidation of glutamine or asparagine, arginine citrullination and methylation. C8-A and C8-B contain each two mass isoforms termed C8-A(H), C8-A(L), C8-B(H) and C8-B(L), (H) standing for higher and (L) for lower molecular weight. C3, C4 and C5 are phosphorylated. The ratio of methylated arginine residues decreases during aging, making the protein more cationic.
    The N-terminal alanine is acetylated (isoform 3, isoform 4, isoform 5 and isoform 6).
    Arg-241 was found to be 6% monomethylated and 60% symmetrically dimethylated.
  • Cellular localizationMyelin membrane. Cytoplasmic side of myelin.
  • Information by UniProt
  • Database links
  • Alternative names
    • GDB antibody
    • Golli MBP antibody
    • Golli MBP; myelin basic protein antibody
    • Hemopoietic MBP antibody
    • HMBPR antibody
    • HUGO antibody
    • MBP antibody
    • MBP_CAVPO antibody
    • MBP_HUMAN antibody
    • MGC99675 antibody
    • MLD antibody
    • Myelin A1 protein antibody
    • Myelin A1 Protein, basic antibody
    • Myelin basic protein antibody
    • Myelin Deficient antibody
    • Myelin membrane encephalitogenic protein antibody
    • OTTHUMP00000163776 antibody
    • OTTHUMP00000174387 antibody
    • OTTHUMP00000174388 antibody
    • SHI antibody
    • Shiverer antibody
    • SP antibody
    see all

Anti-Myelin Basic Protein antibody [7G7] images

  • Anti-Myelin Basic Protein antibody [7G7] (ab78156) at 1/10000 dilution + Crude rat spinal cord homogenate

    Predicted band size : 33 kDa
    Observed band size : 18,21.5 kDa (why is the actual band size different from the predicted?)
  • ab78156 (red), at 1/1000 dilution, staining Myelin Basic Protein in rat mixed neuron/glial cultures. Blue is a DNA stain. ab78156 stains an oligodendrocyte and some membrane shed from this cell. Other cells in the field include neurons, astrocytes, microglia and fibroblasts, all of which are completely negative.
  • Lane 1 : Anti-Myelin Basic Protein antibody [7G7] (ab78156) at 1/10000 dilution
    Lane 2 : Anti-Myelin Basic Protein antibody [7D2] (ab78157) at 1/10000 dilution

    Lane 1 : Crude rat spinal cord homogenate
    Lane 2 : Crude rat spinal cord homogenate


    Predicted band size : 33 kDa
    Observed band size : 18,21.5 kDa (why is the actual band size different from the predicted?)
  • Overlay histogram showing SH-SY5Y cells stained with ab78156 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab78156, 1/50 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

References for Anti-Myelin Basic Protein antibody [7G7] (ab78156)

This product has been referenced in:
  • Ravera S  et al. Tricarboxylic acid cycle-sustained oxidative phosphorylation in isolated myelin vesicles. Biochimie 95:1991-8 (2013). ICC/IF . Read more (PubMed: 23851157) »

See 1 Publication for this product

Product Wall

Vorab möchte ich Sie gerne wissen lassen, mit welchenvorläufigen Testergebnissen sich das Labor gemeldet hat:

There are actually 4 different MBP transcripts of size 21.5, 18.5, 17 and 14 in rat, all four of which can be seen in rat ...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"