Anti-Myelin Basic Protein antibody (ab28541)
Key features and details
- Rabbit polyclonal to Myelin Basic Protein
- Suitable for: Sandwich ELISA
- Reacts with: Pig
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
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Overview
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Product name
Anti-Myelin Basic Protein antibody
See all Myelin Basic Protein primary antibodies -
Description
Rabbit polyclonal to Myelin Basic Protein -
Host species
Rabbit -
Specificity
This antibody is specific to myelin basic proteins. It may cross react with other isoforms of myelin basic proteins. -
Tested applications
Suitable for: Sandwich ELISAmore details -
Species reactivity
Reacts with: Pig
Predicted to work with: Mammals -
Immunogen
Full length native protein (purified) corresponding to Cow Myelin Basic Protein.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.00
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.01% Ammonium Acetate -
Concentration information loading...
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Purification notes
The antibody was affinity purified on agarose with an immuno dominant peptide (PRTPPPSQGKGRGL), which is a universal peptide sequence for all isoforms of mammalian myelin basic proteins. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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ELISA pair antibody
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab28541 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Sandwich ELISA |
Use at an assay dependent concentration. To be used as capture antibody. Pair with ab77895 - Rabbit polyclonal to Myelin Basic Protein (HRP)
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Notes |
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Sandwich ELISA
Use at an assay dependent concentration. To be used as capture antibody. Pair with ab77895 - Rabbit polyclonal to Myelin Basic Protein (HRP) |
Target
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Function
The classic group of MBP isoforms (isoform 4-isoform 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non-classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T-cells and neural cells. Differential splicing events combined with optional post-translational modifications give a wide spectrum of isomers, with each of them potentially having a specialized function. Induces T-cell proliferation. -
Tissue specificity
MBP isoforms are found in both the central and the peripheral nervous system, whereas Golli-MBP isoforms are expressed in fetal thymus, spleen and spinal cord, as well as in cell lines derived from the immune system. -
Involvement in disease
Note=The reduction in the surface charge of citrullinated and/or methylated MBP could result in a weakened attachment to the myelin membrane. This mechanism could be operative in demyelinating diseases such as chronical multiple sclerosis (MS), and fulminating MS (Marburg disease). -
Sequence similarities
Belongs to the myelin basic protein family. -
Developmental stage
Expression begins abruptly in 14-16 week old fetuses. Even smaller isoforms seem to be produced during embryogenesis; some of these persisting in the adult. Isoform 4 expression is more evident at 16 weeks and its relative proportion declines thereafter. -
Post-translational
modificationsSeveral charge isomers of MBP; C1 (the most cationic, least modified, and most abundant form), C2, C3, C4, C5, C6, C7, C8-A and C8-B (the least cationic form); are produced as a result of optional PTM, such as phosphorylation, deamidation of glutamine or asparagine, arginine citrullination and methylation. C8-A and C8-B contain each two mass isoforms termed C8-A(H), C8-A(L), C8-B(H) and C8-B(L), (H) standing for higher and (L) for lower molecular weight. C3, C4 and C5 are phosphorylated. The ratio of methylated arginine residues decreases during aging, making the protein more cationic.
The N-terminal alanine is acetylated (isoform 3, isoform 4, isoform 5 and isoform 6).
Arg-241 was found to be 6% monomethylated and 60% symmetrically dimethylated. -
Cellular localization
Myelin membrane. Cytoplasmic side of myelin. - Information by UniProt
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Alternative names
- GDB antibody
- Golli MBP antibody
- Golli MBP; myelin basic protein antibody
see all
Images
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Fixed Immobilized Anti-MBP (ab28541): 2 ug/mL, 100 ul/well
Captured Antigen: Swine MBP , range from 1 to 90 ng/mL
Detection: anti-MBP HRP (ab77895), 0.25 ug/mL in 1% BSA and PBSt -
Immobilized Anti-MBP (ab28541): range from 0.125 to 2 µg/ml, 100 µl/well
Fixed Captured Antigen: Swine MBP, 500 ng/mL
Detection: anti-MBP HRP (ab77895), 0.25 ug/mL in 1% BSA and PBSt
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab28541 has been referenced in 1 publication.
- Li Y et al. Identification of Adrenomedullin-Induced S-Nitrosylated Proteins in JEG-3 Placental Cells. Reprod Sci N/A:N/A (2021). PubMed: 34462873