Overview

  • Product name
    Anti-Myelin Basic Protein antibody
    See all Myelin Basic Protein primary antibodies
  • Description
    Rabbit polyclonal to Myelin Basic Protein
  • Tested applications
    Suitable for: ICC/IF, WB, IHC-FoFr, IHC-Fr, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 150 to the C-terminus of Mouse Myelin Basic Protein.

    (Peptide available as ab40389.)

  • Positive control
    • This antibody gave a positive signal in the following Tissue Lysates: Brain (Mouse) and Brain (Rat).
  • General notes

    For a recombinant version of Myelin Basic Protein antibody (WB, ICC/IF, IHC-P, Human, Mouse, Rat) - see Ab209328 - clone IGX3421

Properties

Applications

Our Abpromise guarantee covers the use of ab40390 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 33 kDa.Can be blocked with Mouse Myelin Basic Protein peptide (ab40389).
IHC-FoFr 1/1000.
IHC-Fr Use at an assay dependent concentration.
IHC-P 1/100 - 1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • Function
    The classic group of MBP isoforms (isoform 4-isoform 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non-classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T-cells and neural cells. Differential splicing events combined with optional post-translational modifications give a wide spectrum of isomers, with each of them potentially having a specialized function. Induces T-cell proliferation.
  • Tissue specificity
    MBP isoforms are found in both the central and the peripheral nervous system, whereas Golli-MBP isoforms are expressed in fetal thymus, spleen and spinal cord, as well as in cell lines derived from the immune system.
  • Involvement in disease
    Note=The reduction in the surface charge of citrullinated and/or methylated MBP could result in a weakened attachment to the myelin membrane. This mechanism could be operative in demyelinating diseases such as chronical multiple sclerosis (MS), and fulminating MS (Marburg disease).
  • Sequence similarities
    Belongs to the myelin basic protein family.
  • Developmental stage
    Expression begins abruptly in 14-16 week old fetuses. Even smaller isoforms seem to be produced during embryogenesis; some of these persisting in the adult. Isoform 4 expression is more evident at 16 weeks and its relative proportion declines thereafter.
  • Post-translational
    modifications
    Several charge isomers of MBP; C1 (the most cationic, least modified, and most abundant form), C2, C3, C4, C5, C6, C7, C8-A and C8-B (the least cationic form); are produced as a result of optional PTM, such as phosphorylation, deamidation of glutamine or asparagine, arginine citrullination and methylation. C8-A and C8-B contain each two mass isoforms termed C8-A(H), C8-A(L), C8-B(H) and C8-B(L), (H) standing for higher and (L) for lower molecular weight. C3, C4 and C5 are phosphorylated. The ratio of methylated arginine residues decreases during aging, making the protein more cationic.
    The N-terminal alanine is acetylated (isoform 3, isoform 4, isoform 5 and isoform 6).
    Arg-241 was found to be 6% monomethylated and 60% symmetrically dimethylated.
  • Cellular localization
    Myelin membrane. Cytoplasmic side of myelin.
  • Information by UniProt
  • Database links
  • Alternative names
    • GDB antibody
    • Golli MBP antibody
    • Golli MBP; myelin basic protein antibody
    • Hemopoietic MBP antibody
    • HMBPR antibody
    • HUGO antibody
    • mbp antibody
    • MBP_CAVPO antibody
    • MBP_HUMAN antibody
    • MGC99675 antibody
    • MLD antibody
    • Myelin A1 protein antibody
    • Myelin A1 Protein, basic antibody
    • Myelin basic protein antibody
    • Myelin Deficient antibody
    • Myelin membrane encephalitogenic protein antibody
    • OTTHUMP00000163776 antibody
    • OTTHUMP00000174387 antibody
    • OTTHUMP00000174388 antibody
    • SHI antibody
    • Shiverer antibody
    • SP antibody
    see all

Images

  • All lanes : Anti-Myelin Basic Protein antibody (ab40390) at 1 µg/ml

    Lane 1 : Brain (Mouse) Tissue Lysate
    Lane 2 : Brain (Rat) Tissue Lysate - normal tissue

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to rabbit IgG - H&L - Pre adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 33 kDa
    Observed band size : 18,23 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 45 kDa. We are unsure as to the identity of these extra bands.This antibody was raised against an immunogen that is predicted to recognize isoforms (5, 7, 8, 10 and 13) of Myelin Basic Protein (MBP). The predicted molecular weights of isoforms (5, 7, 8, 10 and 13) are 18.5kDa, 17kDa, 14kDa, 21kDa and 13kDa respectively.
  • ICC/IF image of ab40390 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab40390, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue).
  • Immunohistochemical detection of Myelin Basic Protein antibody using (ab40390) on PFA perfusion fixed free-floating rat brain sections. Primary antibody used at 1/1000 and incubated for 18 hours @ 20°C in PBS + 0.3 % Triton X100. Secondary antibody: Goat anti-rabbit Alexa Fluor® 488 (1/1000).  Immunostaining is observed widely in tracts of axons, as expected. The pictures show the staining obtained at the level of the cerebral cortex, using the objective X5 (left) or X10 (right). The tissues were perfusion fixed with 4% PFA and later postfixed overnight in the same fixative. They were cryoprotected in 30% sucrose and cut using a cryostat.

    See Abreview

  • ICC/IF image of ab40390 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab40390, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • ab40390 staining rat adult brain saggital tissue section by IHC-P.  Sections were formaldehyde fixed and subjected to heat mediated antigen retrieval in citric acid (pH 6) prior to blocking in 1% BSA for 10 minutes at RT.  The primary antibody was diluted 1/100 and incubated with the sample for 16 hours.  A biotinylated goat anti-rabbit IgG antibody, diluted 1/300, was used as the secondary.

    See Abreview

  • ab40390 staining rat adult brain saggital tissue section by IHC-P.  Sections were formaldehyde fixed and subjected to heat mediated antigen retrieval in citric acid (pH 6) prior to blocking in 1% BSA for 10 minutes at RT.  The primary antibody was diluted 1/100 and incubated with the sample for 16 hours.  A biotinylated goat anti-rabbit IgG antibody, diluted 1/300, was used as the secondary.

    See Abreview

References

This product has been referenced in:
  • Wang N  et al. Collagen scaffold combined with human umbilical cord-derived mesenchymal stem cells promote functional recovery after scar resection in rats with chronic spinal cord injury. J Tissue Eng Regen Med N/A:N/A (2017). Human . Read more (PubMed: 28482124) »
  • Cashman CR & Hoke A Deficiency of adaptive immunity does not interfere with Wallerian degeneration. PLoS One 12:e0177070 (2017). WB ; Mouse . Read more (PubMed: 28475650) »

See all 41 Publications for this product

Customer reviews and Q&As

Application
Western blot
Sample
Human Tissue lysate - whole (Brain)
Gel Running Conditions
Reduced Denaturing (4%-20%)
Loading amount
10 µg
Specification
Brain
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
Username

Mrs. Sandra Braz

Verified customer

Submitted Aug 02 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Sensory neuronal co-culture)
Permeabilization
Yes - 0.5% Triton
Specification
Sensory neuronal co-culture
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 21°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Aug 26 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections ( Mouse Tissue sections -Brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10 mM citrate, pH6.0
Permeabilization
Yes - 0.1% Tween 20 in PBS
Specification
 Mouse Tissue sections -Brain
Blocking step
Serum as blocking agent for 40 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Miss. Chia-Li Lin

Verified customer

Submitted Nov 23 2015

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step
Horse Serum in 0.3% Triton X-100-PBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Antigen retrieval step
None
Sample
Rat Tissue sections (Brain)
Specification
Brain
Permeabilization
No
Fixative
Paraformaldehyde
Username

Brandon Jun

Verified customer

Submitted Dec 17 2014

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer, pH 6
Sample
Mouse Tissue sections (Spinal cord 16 micron cryosection)
Specification
Spinal cord 16 micron cryosection
Permeabilization
Yes - 0.5% Triton X-100
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Sep 08 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Cell lysate - whole cell (prefrontal cortex)
Loading amount
50 µg
Specification
prefrontal cortex
Gel Running Conditions
Reduced Denaturing (12% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Nov 13 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Brain, prefrontal cortex)
Specification
Brain, prefrontal cortex
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Oct 24 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (brain)
Specification
brain
Fixative
Paraformaldehyde
Antigen retrieval step
None
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Mei Deng

Verified customer

Submitted Sep 10 2012

Merci de nous avoir contactés.

Les deux anticorps sont garantis pour fonctionner en IHC (coupes en paraffine ou coupes congelées) et chez la souris.
Personnellement je recommanderais le polyclonal de lapin ab40390 car c'est u...

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Thank you for contacting us.

Myelin basic protein and myelin protein Zero are the common membrane associated proteins. Please check the datasheets

ab7349, ab40390, ab62631, ab53294 etc. and ab31851

ab128836 is an anti myelin ...

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