Anti-Myelin Basic Protein antibody - Carboxyterminal end (ab65988)

Overview

  • Product nameAnti-Myelin Basic Protein antibody - Carboxyterminal end
    See all Myelin Basic Protein primary antibodies
  • Description
    Rabbit polyclonal to Myelin Basic Protein - Carboxyterminal end
  • Tested applicationsSuitable for: WB, IHC-P, ICCmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide corresponding to a sequence at the C-terminal of the human Myelin Basic Protein, identical to the related rat and mouse sequences.

  • Positive control
    • Human liver. Rat brain tissue lysate.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab65988 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 21.5 kDa (predicted molecular weight: 21.5 kDa).
IHC-P Use a concentration of 1 - 2 µg/ml.
ICC Use at an assay dependent concentration.

Target

  • FunctionThe classic group of MBP isoforms (isoform 4-isoform 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non-classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T-cells and neural cells. Differential splicing events combined with optional post-translational modifications give a wide spectrum of isomers, with each of them potentially having a specialized function. Induces T-cell proliferation.
  • Tissue specificityMBP isoforms are found in both the central and the peripheral nervous system, whereas Golli-MBP isoforms are expressed in fetal thymus, spleen and spinal cord, as well as in cell lines derived from the immune system.
  • Involvement in diseaseNote=The reduction in the surface charge of citrullinated and/or methylated MBP could result in a weakened attachment to the myelin membrane. This mechanism could be operative in demyelinating diseases such as chronical multiple sclerosis (MS), and fulminating MS (Marburg disease).
  • Sequence similaritiesBelongs to the myelin basic protein family.
  • Developmental stageExpression begins abruptly in 14-16 week old fetuses. Even smaller isoforms seem to be produced during embryogenesis; some of these persisting in the adult. Isoform 4 expression is more evident at 16 weeks and its relative proportion declines thereafter.
  • Post-translational
    modifications
    Several charge isomers of MBP; C1 (the most cationic, least modified, and most abundant form), C2, C3, C4, C5, C6, C7, C8-A and C8-B (the least cationic form); are produced as a result of optional PTM, such as phosphorylation, deamidation of glutamine or asparagine, arginine citrullination and methylation. C8-A and C8-B contain each two mass isoforms termed C8-A(H), C8-A(L), C8-B(H) and C8-B(L), (H) standing for higher and (L) for lower molecular weight. C3, C4 and C5 are phosphorylated. The ratio of methylated arginine residues decreases during aging, making the protein more cationic.
    The N-terminal alanine is acetylated (isoform 3, isoform 4, isoform 5 and isoform 6).
    Arg-241 was found to be 6% monomethylated and 60% symmetrically dimethylated.
  • Cellular localizationMyelin membrane. Cytoplasmic side of myelin.
  • Information by UniProt
  • Database links
  • Alternative names
    • GDB antibody
    • Golli MBP antibody
    • Golli MBP; myelin basic protein antibody
    • Hemopoietic MBP antibody
    • HMBPR antibody
    • HUGO antibody
    • MBP antibody
    • MBP_CAVPO antibody
    • MBP_HUMAN antibody
    • MGC99675 antibody
    • MLD antibody
    • Myelin A1 protein antibody
    • Myelin A1 Protein, basic antibody
    • Myelin basic protein antibody
    • Myelin Deficient antibody
    • Myelin membrane encephalitogenic protein antibody
    • OTTHUMP00000163776 antibody
    • OTTHUMP00000174387 antibody
    • OTTHUMP00000174388 antibody
    • SHI antibody
    • Shiverer antibody
    • SP antibody
    see all

Anti-Myelin Basic Protein antibody - Carboxyterminal end images

  • ab65988, at 1µg/ml, staining Myelin Basic Protein in paraffin-embedded human liver tissue by immunohistochemistry.
  • Anti-Myelin Basic Protein antibody - Carboxyterminal end (ab65988) at 1 µg/ml + Rat Brain tissue lysate

    Predicted band size : 21.5 kDa
    Observed band size : 21.5 kDa
    Additional bands at : 25 kDa. We are unsure as to the identity of these extra bands.

References for Anti-Myelin Basic Protein antibody - Carboxyterminal end (ab65988)

This product has been referenced in:
  • Kohli N  et al. Oral delivery of bioencapsulated proteins across blood-brain and blood-retinal barriers. Mol Ther 22:535-46 (2014). Mouse, Human . Read more (PubMed: 24281246) »

See 1 Publication for this product

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