Recombinant
RabMAb

Anti-Myelin Basic Protein antibody [EPR10144] (ab155995)

Overview

  • Product name
    Anti-Myelin Basic Protein antibody [EPR10144]
    See all Myelin Basic Protein primary antibodies
  • Description
    Rabbit monoclonal [EPR10144] to Myelin Basic Protein
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
    Unsuitable for: ICC,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment corresponding to Human Myelin Basic Protein (Uniprot P02686).

  • Positive control
    • Mouse brain, rat brain and Human fetal lung lysates;
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab155995 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 19-33 kDa.
  • Application notes
    Is unsuitable for ICC,IHC-P or IP.
  • Target

    • Function
      The classic group of MBP isoforms (isoform 4-isoform 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non-classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T-cells and neural cells. Differential splicing events combined with optional post-translational modifications give a wide spectrum of isomers, with each of them potentially having a specialized function. Induces T-cell proliferation.
    • Tissue specificity
      MBP isoforms are found in both the central and the peripheral nervous system, whereas Golli-MBP isoforms are expressed in fetal thymus, spleen and spinal cord, as well as in cell lines derived from the immune system.
    • Involvement in disease
      Note=The reduction in the surface charge of citrullinated and/or methylated MBP could result in a weakened attachment to the myelin membrane. This mechanism could be operative in demyelinating diseases such as chronical multiple sclerosis (MS), and fulminating MS (Marburg disease).
    • Sequence similarities
      Belongs to the myelin basic protein family.
    • Developmental stage
      Expression begins abruptly in 14-16 week old fetuses. Even smaller isoforms seem to be produced during embryogenesis; some of these persisting in the adult. Isoform 4 expression is more evident at 16 weeks and its relative proportion declines thereafter.
    • Post-translational
      modifications
      Several charge isomers of MBP; C1 (the most cationic, least modified, and most abundant form), C2, C3, C4, C5, C6, C7, C8-A and C8-B (the least cationic form); are produced as a result of optional PTM, such as phosphorylation, deamidation of glutamine or asparagine, arginine citrullination and methylation. C8-A and C8-B contain each two mass isoforms termed C8-A(H), C8-A(L), C8-B(H) and C8-B(L), (H) standing for higher and (L) for lower molecular weight. C3, C4 and C5 are phosphorylated. The ratio of methylated arginine residues decreases during aging, making the protein more cationic.
      The N-terminal alanine is acetylated (isoform 3, isoform 4, isoform 5 and isoform 6).
      Arg-241 was found to be 6% monomethylated and 60% symmetrically dimethylated.
    • Cellular localization
      Myelin membrane. Cytoplasmic side of myelin.
    • Information by UniProt
    • Database links
    • Alternative names
      • GDB antibody
      • Golli MBP antibody
      • Golli MBP; myelin basic protein antibody
      • Hemopoietic MBP antibody
      • HMBPR antibody
      • HUGO antibody
      • MBP antibody
      • MBP_CAVPO antibody
      • MBP_HUMAN antibody
      • MGC99675 antibody
      • MLD antibody
      • Myelin A1 protein antibody
      • Myelin A1 Protein, basic antibody
      • Myelin basic protein antibody
      • Myelin Deficient antibody
      • Myelin membrane encephalitogenic protein antibody
      • OTTHUMP00000163776 antibody
      • OTTHUMP00000174387 antibody
      • OTTHUMP00000174388 antibody
      • SHI antibody
      • Shiverer antibody
      • SP antibody
      see all

    Images

    • All lanes : Anti-Myelin Basic Protein antibody [EPR10144] (ab155995) at 1/1000 dilution

      Lane 1 : Mouse brain lysate
      Lane 2 : Rat brain lysate
      Lane 3 : Human fetal lung lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 19-33 kDa

    • Anti-Myelin Basic Protein antibody [EPR10144] (ab155995) at 1/1000 dilution + Human GST/HIS-tagged Myelin Basic Protein recombinant protein at 0.01 µg

      Secondary
      Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 19-33 kDa

    References

    ab155995 has not yet been referenced specifically in any publications.

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