Anti-Myelin Basic Protein antibody [MBP101] (ab62631)
- Datasheet
- Specific References (20)
- Protocols
Overview
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Product nameAnti-Myelin Basic Protein antibody [MBP101]
See all Myelin Basic Protein primary antibodies -
DescriptionMouse monoclonal [MBP101] to Myelin Basic Protein
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Tested applicationsSuitable for: WB, ELISA, IHC-P, IHC-FoFr, ICC/IF, Flow Cyt, IHC-Frmore details
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Species reactivityReacts with: Mouse, Rat, Sheep, Rabbit, Goat, Human
Predicted to work with: Non human primates
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Immunogen
Purified human myelin basic protein.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Storage bufferPreservative: None
Constituents: PBS, pH 7.4 -
Concentration information loading... -
PurityProtein G purified
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Purification notesPurified antibody
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ClonalityMonoclonal
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Clone numberMBP101
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IsotypeIgG2b
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Research areas
Associated products
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Compatible Secondaries
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Immunohistochemistry kits
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Isotype control
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Related Products
Applications
Our Abpromise guarantee covers the use of ab62631 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use at an assay dependent concentration. Predicted molecular weight: 33 kDa. | |
| ELISA | Use at an assay dependent concentration. | |
| IHC-P | Use at an assay dependent concentration. PubMed: 23700462 | |
| IHC-FoFr | Use at an assay dependent concentration. PubMed: 24062649 | |
| ICC/IF | 1/500. | |
| Flow Cyt | Use 1µg for 106 cells. ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
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| IHC-Fr | Use at an assay dependent concentration. |
Target
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FunctionThe classic group of MBP isoforms (isoform 4-isoform 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non-classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T-cells and neural cells. Differential splicing events combined with optional post-translational modifications give a wide spectrum of isomers, with each of them potentially having a specialized function. Induces T-cell proliferation.
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Tissue specificityMBP isoforms are found in both the central and the peripheral nervous system, whereas Golli-MBP isoforms are expressed in fetal thymus, spleen and spinal cord, as well as in cell lines derived from the immune system.
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Involvement in diseaseNote=The reduction in the surface charge of citrullinated and/or methylated MBP could result in a weakened attachment to the myelin membrane. This mechanism could be operative in demyelinating diseases such as chronical multiple sclerosis (MS), and fulminating MS (Marburg disease).
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Sequence similaritiesBelongs to the myelin basic protein family.
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Developmental stageExpression begins abruptly in 14-16 week old fetuses. Even smaller isoforms seem to be produced during embryogenesis; some of these persisting in the adult. Isoform 4 expression is more evident at 16 weeks and its relative proportion declines thereafter.
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Post-translational
modificationsSeveral charge isomers of MBP; C1 (the most cationic, least modified, and most abundant form), C2, C3, C4, C5, C6, C7, C8-A and C8-B (the least cationic form); are produced as a result of optional PTM, such as phosphorylation, deamidation of glutamine or asparagine, arginine citrullination and methylation. C8-A and C8-B contain each two mass isoforms termed C8-A(H), C8-A(L), C8-B(H) and C8-B(L), (H) standing for higher and (L) for lower molecular weight. C3, C4 and C5 are phosphorylated. The ratio of methylated arginine residues decreases during aging, making the protein more cationic.
The N-terminal alanine is acetylated (isoform 3, isoform 4, isoform 5 and isoform 6).
Arg-241 was found to be 6% monomethylated and 60% symmetrically dimethylated. -
Cellular localizationMyelin membrane. Cytoplasmic side of myelin.
- Information by UniProt
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Database links
- Entrez Gene: 4155 Human
- Entrez Gene: 17196 Mouse
- Entrez Gene: 24547 Rat
- Omim: 159430 Human
- SwissProt: P02686 Human
- SwissProt: P04370 Mouse
- SwissProt: P02688 Rat
- Unigene: 551713 Human
see all -
Alternative names
- GDB antibody
- Golli MBP antibody
- Golli MBP; myelin basic protein antibody
see all
Anti-Myelin Basic Protein antibody [MBP101] images
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](http://a.abcam.com/ps/products/62/ab62631/Images/Myelin-Basic-Protein-Primary-antibodies-ab62631-5.jpg)
Flow Cytometry-Anti-Myelin Basic Protein antibody [MBP101](ab62631)Overlay histogram showing SH-SY5Y cells stained with ab62631 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab62631, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions. -
![Immunocytochemistry/ Immunofluorescence - Anti-Myelin Basic Protein antibody [MBP101] (ab62631)](http://a.abcam.com/ps/products/62/ab62631/Images/Myelin-Basic-Protein-Primary-antibodies-ab62631-3.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Myelin Basic Protein antibody [MBP101] (ab62631)This image is courtesy of an abreview submitted by Ruma Raha-Chowdhury, University Of Cambridge, United KingdomICC/IF image of Rat Oligodendrocytes primary culture stained with ab62631. The cells on cover slip were incubated in 10% normal donkey serum in 0.1% PBS- and triton X100 for 1h to permeabilise the cells and block non-specific protein-protein interactions. The sections were then incubated with the antibody (ab62631, 2µg/ml) overnight at +4°C. The secondary antibody was Alexa Fluor®568 donkey anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Basic Protein antibody [MBP101] (ab62631)](http://a.abcam.com/ps/products/62/ab62631/Images/ab62631-260979-ab62631IHC.JPG)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Basic Protein antibody [MBP101] (ab62631)ab62631 staining MBP in Mouse brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with normal serum of species secondary antibody; antigen retrieval was by heat mediation in 10mM citric acid buffer. Samples were incubated with primary antibody (1/200) overnight at 4°C.
Protocols
References for Anti-Myelin Basic Protein antibody [MBP101] (ab62631)
This product has been referenced in:
- Satoh JI et al. Expression of GPR17, a regulator of oligodendrocyte differentiation and maturation, in Nasu-Hakola disease brains. Intractable Rare Dis Res 6:50-54 (2017). IHC-P ; Human . Read more (PubMed: 28357182) »
- Fujita Y et al. Decreased cohesin in the brain leads to defective synapse development and anxiety-related behavior. J Exp Med 214:1431-1452 (2017). IHC-P ; Human . Read more (PubMed: 28408410) »
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"