Purified human myelin basic protein.
Our Abpromise guarantee covers the use of ab62631 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | Use at an assay dependent concentration. Predicted molecular weight: 33 kDa. | |
ELISA | Use at an assay dependent concentration. | |
IHC-P | Use at an assay dependent concentration. PubMed: 23700462 | |
IHC-FoFr | Use at an assay dependent concentration. PubMed: 24062649 | |
ICC/IF | 1/500. | |
Flow Cyt | Use 1µg for 106 cells. ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
|
IHC-Fr | Use at an assay dependent concentration. |
ICC/IF image of Rat Oligodendrocytes primary culture stained with ab62631. The cells on cover slip were incubated in 10% normal donkey serum in 0.1% PBS- and triton X100 for 1h to permeabilise the cells and block non-specific protein-protein interactions. The sections were then incubated with the antibody (ab62631, 2µg/ml) overnight at +4°C. The secondary antibody was Alexa Fluor®568 donkey anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab62631 staining MBP in Mouse brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with normal serum of species secondary antibody; antigen retrieval was by heat mediation in 10mM citric acid buffer. Samples were incubated with primary antibody (1/200) overnight at 4°C.
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