• Product nameAnti-Myelin Basic Protein antibody [QD-9]
    See all Myelin Basic Protein primary antibodies
  • Description
    Mouse monoclonal [QD-9] to Myelin Basic Protein
  • SpecificityThis antibody can specifically detect degenerating myelin (demyelinating lesions) in brains with multiple sclerosis, multiple system atrophy (MSA), as well as infarcted brains. ab22460 is, therefore, a very useful tool for detecting demyelination but not myelin in normal brain.
  • Tested applicationsSuitable for: WB, IHC-FoFrmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide:


    , corresponding to amino acids 82-88 of the short form of Human Myelin Basic Protein.

  • General notesFor maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.



Our Abpromise guarantee covers the use of ab22460 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution. Detects a band of approximately 18.5 kDa (predicted molecular weight: 22 kDa).
IHC-FoFr Use at an assay dependent dilution.


  • FunctionThe classic group of MBP isoforms (isoform 4-isoform 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non-classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T-cells and neural cells. Differential splicing events combined with optional post-translational modifications give a wide spectrum of isomers, with each of them potentially having a specialized function. Induces T-cell proliferation.
  • Tissue specificityMBP isoforms are found in both the central and the peripheral nervous system, whereas Golli-MBP isoforms are expressed in fetal thymus, spleen and spinal cord, as well as in cell lines derived from the immune system.
  • Involvement in diseaseNote=The reduction in the surface charge of citrullinated and/or methylated MBP could result in a weakened attachment to the myelin membrane. This mechanism could be operative in demyelinating diseases such as chronical multiple sclerosis (MS), and fulminating MS (Marburg disease).
  • Sequence similaritiesBelongs to the myelin basic protein family.
  • Developmental stageExpression begins abruptly in 14-16 week old fetuses. Even smaller isoforms seem to be produced during embryogenesis; some of these persisting in the adult. Isoform 4 expression is more evident at 16 weeks and its relative proportion declines thereafter.
  • Post-translational
    Several charge isomers of MBP; C1 (the most cationic, least modified, and most abundant form), C2, C3, C4, C5, C6, C7, C8-A and C8-B (the least cationic form); are produced as a result of optional PTM, such as phosphorylation, deamidation of glutamine or asparagine, arginine citrullination and methylation. C8-A and C8-B contain each two mass isoforms termed C8-A(H), C8-A(L), C8-B(H) and C8-B(L), (H) standing for higher and (L) for lower molecular weight. C3, C4 and C5 are phosphorylated. The ratio of methylated arginine residues decreases during aging, making the protein more cationic.
    The N-terminal alanine is acetylated (isoform 3, isoform 4, isoform 5 and isoform 6).
    Arg-241 was found to be 6% monomethylated and 60% symmetrically dimethylated.
  • Cellular localizationMyelin membrane. Cytoplasmic side of myelin.
  • Information by UniProt
  • Database links
  • Alternative names
    • GDB antibody
    • Golli MBP antibody
    • Golli MBP; myelin basic protein antibody
    • Hemopoietic MBP antibody
    • HMBPR antibody
    • HUGO antibody
    • MBP antibody
    • MBP_HUMAN antibody
    • MGC99675 antibody
    • MLD antibody
    • Myelin A1 protein antibody
    • Myelin basic protein antibody
    • Myelin Deficient antibody
    • Myelin membrane encephalitogenic protein antibody
    • OTTHUMP00000163776 antibody
    • OTTHUMP00000174387 antibody
    • OTTHUMP00000174388 antibody
    • SHI antibody
    • Shiverer antibody
    • SP antibody
    see all

Anti-Myelin Basic Protein antibody [QD-9] images

  • Anti-Myelin Basic Protein antibody [QD-9] (ab22460) at 1/50000 dilution + Extracts of multiple system atrophy (MSA) brain homogenates

    Appropriate alkaline phosphatase labelled antibody at 1/1000 dilution

    Predicted band size : 22 kDa
    Observed band size : 17.2,18.5 kDa (why is the actual band size different from the predicted?)
  • Double staining with ab22460 (approx. 1/30000 dilution) and anti-ubiquitin in a multiple system atrophy (MSA) case (moderately affected cerebellar medulla). The distribution of ab22460 (purple) is different from that of ubiquinated glial cytoplasmic inclusions (GCIs,brown). Note that ab22460 antibody does not stain the GCIs.

References for Anti-Myelin Basic Protein antibody [QD-9] (ab22460)

This product has been referenced in:
  • Taghipour Z  et al. Transplantation of Undifferentiated and Induced Human Exfoliated Deciduous Teeth-derived Stem Cells Promote Functional Recovery of Rat Spinal Cord Contusion Injury Model. Stem Cells Dev : (2011). IHC-FoFr ; Rat . Read more (PubMed: 21970342) »

See 1 Publication for this product

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