The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 64 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
RelevanceMyelin expression factor 2 (MEF2) is expressed early in the differentiation program and is suppressed by specific polypeptide growth factors. The ability of MEF2 to recognize conserved activating elements associated with multiple specific genes suggests that this factor may participate in the coordinate regulation of genes during myogenesis.