Anti-Myelin oligodendrocyte glycoprotein antibody (ab32760)

Overview

  • Product name
    Anti-Myelin oligodendrocyte glycoprotein antibody
    See all Myelin oligodendrocyte glycoprotein primary antibodies
  • Description
    Rabbit polyclonal to Myelin oligodendrocyte glycoprotein
  • Tested applications
    Suitable for: WB, IHC-FoFr, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat
    Predicted to work with: Cow, Human, Common marmoset
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 200 to the C-terminus of Rat Myelin oligodendrocyte glycoprotein.

    (Peptide available as ab32759.)

  • Positive control
    • Mouse Brain Whole Tissue Lysate and Rat Brain Whole Tissue Lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab32760 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 27 kDa (predicted molecular weight: 27 kDa).Can be blocked with Rat Myelin oligodendrocyte glycoprotein peptide (ab32759).
IHC-FoFr Use at an assay dependent concentration. PubMed: 22269040
IP Use a concentration of 5 µg/ml.

Target

Images

  • All lanes : Anti-Myelin oligodendrocyte glycoprotein antibody (ab32760) at 1 µg/ml

    Lane 1 : Mouse Brain Whole Tissue Lysate
    Lane 2 : Rat Brain Whole Tissue Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    IR Dye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution

    Performed under reducing conditions.

    Predicted band size : 27 kDa
    Observed band size : 27 kDa
  • Ab32760 used on Mouse Cortex and Hippocampus in free floating sections using direct fluorescence. The tissues were fixed (animals perfused fixed) with 4% PFA and later postfixed overnight in the same fixative. They were cryoprotected in 30% sucrose and cut using a cryostat.

    See Abreview

  • Myelin oligodendrocyte glycoprotein was immunoprecipitated using 0.5mg Mouse Brain tissue lysate, 5µg of Rabbit polyclonal to Myelin oligodendrocyte glycoprotein and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, Mouse Brain tissue lysate lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab32760.
    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
    Band: 27kDa: Myelin oligodendrocyte glycoprotein

References

This product has been referenced in:
  • Dai J  et al. Olig1 function is required for oligodendrocyte differentiation in the mouse brain. J Neurosci 35:4386-402 (2015). WB ; Mouse . Read more (PubMed: 25762682) »
  • Wahl SE  et al. Mammalian target of rapamycin promotes oligodendrocyte differentiation, initiation and extent of CNS myelination. J Neurosci 34:4453-65 (2014). Read more (PubMed: 24671992) »

See all 5 Publications for this product

Customer reviews and Q&As

Application
Western blot
Sample
Rat Tissue lysate - whole (liver and brain)
Gel Running Conditions
Reduced Denaturing (12.5%)
Loading amount
50 µg
Specification
liver and brain
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Nov 13 2017

Thank you so much for your call today and letting us know about the trouble with the vial of ab32760.

As we discussed, I'm sending a free of charge vial of the antibody on the order ***, which should arrive tomorrow.

Please keepme u...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (brain sections)
Specification
brain sections
Fixative
Paraformaldehyde
Antigen retrieval step
None
Permeabilization
No
Username

Dr. Sophie Pezet

Verified customer

Submitted Apr 27 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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