Recombinant
RabMAb

Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281] (ab109746)

Overview

  • Product name
    Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281]
    See all Myelin oligodendrocyte glycoprotein primary antibodies
  • Description
    Rabbit monoclonal [EP4281] to Myelin oligodendrocyte glycoprotein
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Myelin oligodendrocyte glycoprotein aa 50-150 (extracellular). The exact sequence is proprietary.

  • Positive control
    • Mouse brain, Rat brain and Human brain lysates; Human astrocytoma tissue and Human brain tissue
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab109746 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 28 kDa.
IHC-P 1/1000 - 1/5000.

See IHC antigen retrieval protocols.

Target

  • Function
    Mediates homophilic cell-cell adhesion (By similarity). Minor component of the myelin sheath. May be involved in completion and/or maintenance of the myelin sheath and in cell-cell communication.
  • Tissue specificity
    Found exclusively in the CNS, where it is localized on the surface of myelin and oligodendrocyte cytoplasmic membranes.
  • Sequence similarities
    Belongs to the immunoglobulin superfamily. BTN/MOG family.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • BTN6 antibody
    • BTNL11 antibody
    • MGC26137 antibody
    • MOG alpha 5 antibody
    • MOG alpha 6 antibody
    • MOG AluA antibody
    • MOG AluB antibody
    • MOG antibody
    • MOG Ig AluB antibody
    • MOG_HUMAN antibody
    • MOGIG2 antibody
    • Myelin oligodendrocyte glycoprotein antibody
    • Myelin-oligodendrocyte glycoprotein antibody
    • NRCLP7 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat cerebrum tissue sections labeling Myelin oligodendrocyte glycoprotein with purified ab109746 at 1:1000 dilution (1.12 μg/ml). Heat mediated antigen retrieval was performed using Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use). PBS instead of the primary antibody was used as the negative control.

  • All lanes : Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281] (ab109746) at 1/1000 dilution

    Lane 1 : Mouse brain lysate
    Lane 2 : Rat brain lysate
    Lane 3 : Human brain lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 28 kDa



    This image was produced using unpurified antibody.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse cerebrum tissue sections labeling Myelin oligodendrocyte glycoprotein with Purified ab109746 at 1:1000 dilution (1.12 μg/ml). Heat mediated antigen retrieval was performed using Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cerebrum tissue sections labeling Myelin oligodendrocyte glycoprotein with purified ab109746 at 1:1000 dilution (1.12 μg/ml). Heat mediated antigen retrieval was performed using Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use). PBS instead of the primary antibody was used as the negative control.

  • Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281] (ab109746) at 1/2000 dilution (Purified) + Mouse brain lysates at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 28 kDa
    Observed band size: 28 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human astrocytoma tissue sections labeling Myelin oligodendrocyte glycoprotein with purified ab109746 at 1:1000 dilution (1.12 μg/ml). Heat mediated antigen retrieval was performed using Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use). PBS instead of the primary antibody was used as the negative control.

  • ab109746, at 1/1000 dilution, staining Myelin oligodendrocyte glycoprotein in paraffin-embedded Human astrocytoma tissue by Immunohistochemistry. This image was produced using unpurified antibody.

  • All lanes : Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281] (ab109746) at 1/2000 dilution

    Lane 1 : Human brain lysates
    Lane 2 : Rat brain lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 28 kDa
    Observed band size: 28 kDa

  • ab109746, at 1/1000 dilution, staining Myelin oligodendrocyte glycoprotein in paraffin-embedded Human brain tissue by Immunohistochemistry. This image was produced using unpurified antibody.

  • ab109746 showing negative staining in Breast carcinoma tissue. This image was produced using unpurified antibody.

  • ab109746 showing negative staining in Normal breast tissue. This image was produced using unpurified antibody.

  • ab109746 showing positive staining in Glioblastoma tissue. This image was produced using unpurified antibody.

  • ab109746 showing negative staining in Colonic adenocarcinoma tissue. This image was produced using unpurified antibody.

  • ab109746 showing negative staining in Normal tonsil tissue. This image was produced using unpurified antibody.

  • ab109746 showing negative staining in Normal colon tissue. This image was produced using unpurified antibody.

  • ab109746 showing negative staining in Benign prostatic hyperplasia tissue. This image was produced using unpurified antibody.

References

This product has been referenced in:
  • Jana M  et al. Upregulation of Myelin Gene Expression by a Physically-Modified Saline via Phosphatidylinositol 3-Kinase-Mediated Activation of CREB: Implications for Multiple Sclerosis. Neurochem Res 43:407-419 (2018). Read more (PubMed: 29143164) »
  • Kim MJ  et al. The Anti-Inflammatory Effects of Oral-Formulated Tacrolimus in Mice with Experimental Autoimmune Encephalomyelitis. J Korean Med Sci 32:1502-1507 (2017). Read more (PubMed: 28776347) »

See all 8 Publications for this product

Customer reviews and Q&As

Application
Western blot
Sample
Rat Tissue lysate - whole (liver and brain)
Gel Running Conditions
Reduced Denaturing (12.5%)
Loading amount
50 µg
Specification
liver and brain
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Nov 13 2017

Application
Western blot
Sample
Mouse Tissue lysate - whole (liver and brain)
Gel Running Conditions
Reduced Denaturing (4-20)
Loading amount
10 µg
Specification
liver and brain
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Isabelle Cote

Verified customer

Submitted Sep 28 2017

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Primary oligodendrocytes differentiated for 5 days)
Permeabilization
No
Specification
Primary oligodendrocytes differentiated for 5 days
Blocking step
Serum as blocking agent for 10 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted May 05 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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