Anti-Myelin PLP antibody (ab105784)

Overview

  • Product nameAnti-Myelin PLP antibody
    See all Myelin PLP primary antibodies
  • Description
    Rabbit polyclonal to Myelin PLP
  • Tested applicationsSuitable for: WB, IHC-P, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Goat, Cat, Human, Xenopus laevis, Marmoset (common)
    Predicted to work with: Rabbit, Chicken, Cow, Dog, Pig, Orangutan
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Rat Myelin PLP.

  • Positive control
    • This antibody gave a positive signal in Rat Brain and Rat Spinal Cord tissue lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab105784 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 34 kDa (predicted molecular weight: 30 kDa).
IHC-P 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IHC-Fr 1/2000.

Target

  • FunctionThis is the major myelin protein from the central nervous system. It plays an important role in the formation or maintenance of the multilamellar structure of myelin.
  • Involvement in diseaseDefects in PLP1 are the cause of leukodystrophy hypomyelinating type 1 (HLD1) [MIM:312080]; also known as Pelizaeus-Merzbacher disease. HLD1 is an X-linked recessive dysmyelinating disorder of the central nervous system in which myelin is not formed properly. It is characterized clinically by nystagmus, spastic quadriplegia, ataxia, and developmental delay.
    Defects in PLP1 are the cause of spastic paraplegia X-linked type 2 (SPG2) [MIM:312920]. SPG2 is characterized by spastic gait and hyperreflexia. In some patients, complicating features include nystagmus, dysarthria, sensory disturbance, mental retardation, optic atrophy.
  • Sequence similaritiesBelongs to the myelin proteolipid protein family.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • HLD1 antibody
    • Lipophilin antibody
    • Major myelin proteolipid protein antibody
    • MMPL antibody
    • Myelin proteolipid protein antibody
    • MYPR_HUMAN antibody
    • PLP 1 antibody
    • PLP antibody
    • PLP/DM20 antibody
    • PLP1 antibody
    • PMD antibody
    • Proteolipid protein 1 antibody
    • SPG2 antibody
    see all

Anti-Myelin PLP antibody images

  • IHC-Fr image of myelin PLP staining on Mouse sciatic nerve sections using ab105784 (1:3000). Fresh frozen sections (10μM) and dried in fumehood for 2 hours before fixing with 10% formalin for 10 min. Sections were permeablized in 0.1% Triton X-100 in TBS for 10min. Sections were then blocked using 1% BSA for 10 mins at 21°C. ab105784 was diluted 1:2000 in TBS buffer (containing BSA) and sections were then incubated with ab105784 for 16 hours at 21°C. The secondary antibody used was Alexa Fluor® 594 conjugated Goat polyclonal to Mouse IgG (1:1000).

    See Abreview

  • ab105784 staining rat adult brain sections by IHC-P. The tissue was fixed with formaldehyde and a heat mediated antigen retrival step was performed with citric acid pH 6. Blocking of the sample was done with 1%BSA for 10 minutes at 21°C, followed by staining with ab105784 at 1/1000 in TBS/BSA/azide for 1h at 21°C. A biotinylated goat anti-rabbit polyclonal antibody at 1/200 was used as the secondary antibody.

    See Abreview

  • All lanes : Anti-Myelin PLP antibody (ab105784) at 1 µg/ml

    Lane 1 : Brain (Rat) Tissue Lysate
    Lane 2 : Spinal Cord (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 30 kDa
    Observed band size : 34 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 minute
  • ab105784 staining human adult brainstem sections by IHC-P. The tissue was fixed with formaldehyde and a heat mediated antigen retrival step was performed with citric acid pH 6. Blocking of the sample was done with 1%BSA for 10 minutes at 21°C, followed by staining with ab105784 at 1/2000 in TBS/BSA/azide for 2h at 21°C. A biotinylated goat anti-rabbit polyclonal antibody at 1/200 was used as the secondary antibody.

    See Abreview

  • ab105784 staining goat cerebellum sections by IHC-P. The tissue was fixed with formaldehyde and a heat mediated antigen retrival step was performed with citric acid pH 6. Blocking of the sample was done with 1%BSA for 10 minutes at 21°C, followed by staining with ab105784 at 1/1000 in TBS/BSA/azide for 2h at 21°C. A biotinylated goat anti-rabbit polyclonal antibody at 1/200 was used as the secondary antibody.

    See Abreview

  • ab105784 staining mouse adult brain sections (grey matter, frontal cortex sagittal plane) by IHC-P. The tissue was fixed with formaldehyde and a heat mediated antigen retrival step was performed with citric acid pH 6. Blocking of the sample was done with 1%BSA for 10 minutes at 21°C, followed by staining with ab105784 at 1/750 in TBS/BSA/azide for 1h at 21°C. A biotinylated goat anti-rabbit polyclonal antibody at 1/200 was used as the secondary antibody.)

    See Abreview

References for Anti-Myelin PLP antibody (ab105784)

ab105784 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry
Sample Rat Cultured Cells (Subventricular zone neurospheres)
Permeabilization No
Specification Subventricular zone neurospheres
Blocking step BSA as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Jul 14 2015

Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Sample Mouse Tissue sections (Sciatic nerve)
Specification Sciatic nerve
Permeabilization Yes - 0.1% TRITON X-100 in TBS
Fixative Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

Submitted May 31 2013

Thank you for your phone call.

DISCOUNT CODE: xxx for ab66043
DISCOUNT CODE: xxx for ab105784
Expiration date: xxx

I am very pleased to hear you would like to accept our offer and test ab66043 and ab105784 in dog. Each co...

Read More

Thank you for your reply and I am sorry that we were not able to resolve the issue that you were having with our antibodies.As requested, I have credited back the cost of the original purchase.

Your credit note ID is *

I have asked ou...

Read More

Thank you for your reply and for providing that information.

Both the ab29486 and ab105784 are giving the same staining pattern which is strange and so I was wandering if you had used a secondary only control on these samples. I am just curiou...

Read More

Thank you for your reply.

Hopefully the new antibody will help clear up the number of bands that you are seeing. You should receive the antibody tomorrow morning by 10.30am and your new order number (for tracking purposes only) is **********....

Read More

Thank you for your reply.

As you are not getting the correct signal in western blot using the antibody against rat samples, then I am happy to send you a replacement antibody free of charge.

The only other antibody in our catalogue ...

Read More

Thank you for that information.

I am sending you ab29475 to use as a positive control. Your order number is *******.

Please let me know how the positive control works out and if there is anything else I can help you with.

Thank you for calling Abcam.

I have not been able to determine why you are getting a signal at 25kDa. As we had not tested this antibody on canine tissue, we were not sure how it would react, so there is a very good chance that the band is th...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Xenopus laevis Tissue sections (optic nerve)
Specification optic nerve
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% Triton-X
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted May 03 2012

1-10 of 17 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"