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Our Abpromise guarantee covers the use of ab28486 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 26,30 kDa.|
|IHC-P||Use at an assay dependent concentration.|
|Flow Cyt||Use at an assay dependent concentration. PubMed: 19659692
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
ab28486 staining Myelin PLP in Rabbit brain tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with methanol, permeablized with acetone and blocked with 5% BSA for 1 hour at 37°C. The sample was incubated with primary antibody (1/100) at 4°C for 18 hours. An Alexa Fluor®488-conjugated Goat anti-rabbit polyclonal (1/200) was used as the secondary antibody.
Immunohistochemical staining of Myelin PLP in mouse brain thick section using ab28486 (1/1000 dilution). Staining (m) is limited to myelin tracts and oligodendrocytes.
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