Overview

  • Product nameAnti-Myelin PLP antibody
    See all Myelin PLP primary antibodies
  • Description
    Rabbit polyclonal to Myelin PLP
  • Specificityab28486 recognises PLP
  • Tested applicationsSuitable for: ELISA, WB, ICC, IHC-FoFr, ICC/IF, IHC-P, IHC-Fr, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Human
    Predicted to work with: Cow, Dog, Pig, Macaque Monkey
  • Immunogen

    Synthetic peptide:

    CG KGLSATVTGG QKGRGSRG

    , corresponding to amino acids 109-128 of Mouse Myelin PLP

  • Positive control
    • Mouse brain

Properties

Applications

Our Abpromise guarantee covers the use of ab28486 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/10000.
WB 1/1000. Predicted molecular weight: 26,30 kDa.
ICC 1/1000.
IHC-FoFr 1/100.
ICC/IF 1/100.
IHC-P Use at an assay dependent concentration.
IHC-Fr 1/500.
Flow Cyt Use at an assay dependent concentration. PubMed: 19659692ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • FunctionThis is the major myelin protein from the central nervous system. It plays an important role in the formation or maintenance of the multilamellar structure of myelin.
  • Involvement in diseaseDefects in PLP1 are the cause of leukodystrophy hypomyelinating type 1 (HLD1) [MIM:312080]; also known as Pelizaeus-Merzbacher disease. HLD1 is an X-linked recessive dysmyelinating disorder of the central nervous system in which myelin is not formed properly. It is characterized clinically by nystagmus, spastic quadriplegia, ataxia, and developmental delay.
    Defects in PLP1 are the cause of spastic paraplegia X-linked type 2 (SPG2) [MIM:312920]. SPG2 is characterized by spastic gait and hyperreflexia. In some patients, complicating features include nystagmus, dysarthria, sensory disturbance, mental retardation, optic atrophy.
  • Sequence similaritiesBelongs to the myelin proteolipid protein family.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • HLD1 antibody
    • Lipophilin antibody
    • Major myelin proteolipid protein antibody
    • MMPL antibody
    • Myelin proteolipid protein antibody
    • MYPR_HUMAN antibody
    • PLP 1 antibody
    • PLP antibody
    • PLP/DM20 antibody
    • PLP1 antibody
    • PMD antibody
    • Proteolipid protein 1 antibody
    • SPG2 antibody
    see all

Anti-Myelin PLP antibody images

  • ab28486 staining Myelin PLP in Rabbit brain tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with methanol, permeablized with acetone and blocked with 5% BSA for 1 hour at 37°C. The sample was incubated with primary antibody (1/100) at 4°C for 18 hours. An Alexa Fluor®488-conjugated Goat anti-rabbit polyclonal (1/200) was used as the secondary antibody.

    See Abreview

  • Immunofluorescence analysis of Human brain cells, staining Myelin PLP with ab28486.

    Cells were fixed with formaldehyde and blocked with 10% serum for 20 minutes at room temperature. Samples were incubated with primary antibody (1/400 in diluent) for 16 hours. An AlexaFluor®555-conjugated donkey anti-rabbit polyclonal IgG (1/1000) was used as the secondary antibody.

    See Abreview

  • Immunohistochemical staining of  Myelin PLP in mouse brain thick section using ab28486 (1/1000 dilution). Staining (m) is limited to myelin tracts and oligodendrocytes.

  • ab28486 at a 1/1000 dilution staining mouse brain (white matter) by IHC-P. The tissue was fixed in 10% formalin saline. Antigen retrieval was via hydrated autoclaving at 121°C for 15 minutes followed by 5 minutes in 98%formic acid. The antibody was incubated with the tissue for 16 hours and then bound antibody was detected using a biotinylated goat anti-rabbit IgG Fc.ab28486 at a 1/1000 dilution staining mouse brain (white matter) by IHC-P. The tissue was fixed in 10% formalin saline. Antigen retrieval was via hydrated autoclaving at 121°C for 15 minutes followed by 5 minutes in 98%formic acid. The antibody was incubated with the tissue for 16 hours and then bound antibody was detected using a biotinylated goat anti-rabbit IgG Fc.

    See Abreview

References for Anti-Myelin PLP antibody (ab28486)

This product has been referenced in:
  • Locatelli G  et al. Mature oligodendrocytes actively increase in vivo cytoskeletal plasticity following CNS damage. J Neuroinflammation 12:62 (2015). Read more (PubMed: 25889302) »
  • Lebrun-Julien F  et al. Balanced mTORC1 Activity in Oligodendrocytes Is Required for Accurate CNS Myelination. J Neurosci 34:8432-48 (2014). Read more (PubMed: 24948799) »

See all 10 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 100 µg
Gel Running Conditions Reduced Denaturing (15%)
Sample Rat Tissue lysate - whole (brain tissue)
Specification brain tissue
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Username

Abcam user community

Verified customer

Submitted Sep 16 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Sample Rabbit Tissue sections (brain)
Specification brain
Permeabilization Yes - Acetone
Fixative Methanol
Username

Abcam user community

Verified customer

Submitted Sep 09 2013

Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application ELISA
Sample Human Cell (brain)
Specification brain
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Type Indirect
Username

Dr. Rachel Barker

Verified customer

Submitted Nov 06 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (brain)
Specification brain
Fixative Formaldehyde
Permeabilization Yes - Citrate
Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: RT°C
Username

Dr. Rachel Barker

Verified customer

Submitted Nov 06 2012

Thank you very much for your call today and for letting us know about the trouble with this antibody.

As we discussed, I'm sending a free of charge vial from a new lot on the order ***, which should arrive tomorrow.

Please keep me u...

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Thank you very much for your call today and for letting us know about the trouble with this antibody.

As we discussed, I'm sending a free of charge vial of ab105784 on the order ***, which should arrive tomorrow. Please keep me updated about ...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (C57B/J mouse Vagus Nerve)
Specification C57B/J mouse Vagus Nerve
Fixative Paraformaldehyde
Permeabilization Yes - 0.05% Triton-X100 in PBS
Blocking step 5% BSA + 0.5% goat serum in PBST as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. Kirk McManus

Verified customer

Submitted Mar 01 2012

Thank you for taking the time to complete our questionnaire.

The details you have kindly provided will enable us to investigate this case for you and this is also helpful in our records for monitoring of quality.

Reviewing this cas...

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Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

I would like to reassure you that this antibody is tested and covered by our 6 month guarantee forWB a...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Rat Tissue sections (Spinal cord)
Specification Spinal cord
Fixative Paraformaldehyde
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Dr. Karine Thibault

Verified customer

Submitted Sep 27 2011

1-10 of 13 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"