Anti-Myelin Protein Zero antibody (ab31851)

Overview

  • Product nameAnti-Myelin Protein Zero antibody
    See all Myelin Protein Zero primary antibodies
  • Description
    Rabbit polyclonal to Myelin Protein Zero
  • Tested applicationsSuitable for: WB, IHC-FoFr, IHC-P, ICC/IFmore details
    Unsuitable for: IHC-Fr
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 200 to the C-terminus of Rat Myelin Protein Zero.

    (Peptide available as ab31869.)

  • Positive control
    • Mouse Sciatic Nerve Whole Tissue Lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab31851 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 25 kDa (predicted molecular weight: 27 kDa).
IHC-FoFr Use at an assay dependent concentration.
IHC-P 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100.
  • Application notesIs unsuitable for IHC-Fr.
  • Target

    • FunctionCreation of an extracellular membrane face which guides the wrapping process and ultimately compacts adjacent lamellae.
    • Tissue specificityFound only in peripheral nervous system Schwann cells.
    • Involvement in diseaseDefects in MPZ are the cause of Charcot-Marie-Tooth disease type 1B (CMT1B) [MIM:118200]. CMT1B is a form of Charcot-Marie-Tooth disease, the most common inherited disorder of the peripheral nervous system. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathy or CMT1, and primary peripheral axonal neuropathy or CMT2. Neuropathies of the CMT1 group are characterized by severely reduced nerve conduction velocities (less than 38 m/sec), segmental demyelination and remyelination with onion bulb formations on nerve biopsy, slowly progressive distal muscle atrophy and weakness, absent deep tendon reflexes, and hollow feet.
      Defects in MPZ are the cause of Charcot-Marie-Tooth disease type 2I (CMT2I) [MIM:607677]. CMT2I is a form of Charcot-Marie-Tooth disease, the most common inherited disorder of the peripheral nervous system. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathy or CMT1, and primary peripheral axonal neuropathy or CMT2. Neuropathies of the CMT2 group are characterized by signs of axonal regeneration in the absence of obvious myelin alterations, normal or slightly reduced nerve conduction velocities, and progressive distal muscle weakness and atrophy. CMT2I is characterized by late onset (range 47 to 60 years).
      Defects in MPZ are the cause of Charcot-Marie-Tooth disease type 2J (CMT2J) [MIM:607736]. CMT2J is a form of Charcot-Marie-Tooth disease characterized by the association of axonal peripheral neuropathy with hearing loss and pupillary abnormalities such as Adie pupil. Inheritance is autosomal dominant.
      Defects in MPZ are the cause of Adie pupil (ADIEP) [MIM:103100]. A stationary, benign disorder characterized by tonic, sluggishly reacting pupil and hypoactive or absent tendon reflexes. Adie pupil is a characteristic of Charcot-Marie-Tooth disease type 2J.
      Defects in MPZ may be the cause of Charcot-Marie-Tooth disease dominant intermediate type D (CMTDID) [MIM:607791]. CMTDID is a form of Charcot-Marie-Tooth disease characterized by features intermediate between demyelinating and axonal peripheral neuropathies, and motor median nerve conduction velocities ranging from 25 to 45 m/sec.
      Defects in MPZ are a cause of Dejerine-Sottas syndrome (DSS) [MIM:145900]; also known as Dejerine-Sottas neuropathy (DSN) or hereditary motor and sensory neuropathy III (HMSN3). DSS is a severe degenerating neuropathy of the demyelinating Charcot-Marie-Tooth disease category, with onset by age 2 years. DSS is characterized by motor and sensory neuropathy with very slow nerve conduction velocities, increased cerebrospinal fluid protein concentrations, hypertrophic nerve changes, delayed age of walking as well as areflexia. There are both autosomal dominant and autosomal recessive forms of Dejerine-Sottas syndrome.
      Defects in MPZ are a cause of congenital hypomyelination neuropathy (CHN) [MIM:605253]. CHN is characterized clinically by early onset of hypotonia, areflexia, distal muscle weakness, and very slow nerve conduction velocities.
      Defects in MPZ are a cause of Roussy-Levy syndrome (ROULS) [MIM:180800]; also known as Roussy-Levy hereditary areflexic dystasia. This autosomal dominant disorder resembles Charcot-Marie-Tooth disease type 1 in that it presents with foot deformity, weakness and atrophy of distal limb muscles, especially the peronei, and absent tendon reflexes. The phenotype differs, however, in that it includes static tremor of the upper limbs and gait ataxia.
    • Sequence similaritiesBelongs to the myelin P0 protein family.
      Contains 1 Ig-like V-type (immunoglobulin-like) domain.
    • Post-translational
      modifications
      N-glycosylated; contains sulfate-substituted glycan.
    • Cellular localizationMembrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • Charcot Marie Tooth neuropathy 1B antibody
      • CHM antibody
      • CMT1 antibody
      • CMT1B antibody
      • CMT2I antibody
      • CMT2J antibody
      • CMT4E antibody
      • CMTDI3 antibody
      • CMTDID antibody
      • DSS antibody
      • HMSNIB antibody
      • MPP antibody
      • MPZ antibody
      • Myelin peripheral protein antibody
      • Myelin protein P0 antibody
      • Myelin protein zero antibody
      • MYP0_HUMAN antibody
      • P0 antibody
      see all

    Anti-Myelin Protein Zero antibody images

    • Anti-Myelin Protein Zero antibody (ab31851) at 1 µg/ml + Mouse Sciatic Nerve Whole Tissue Lysate at 20 µg

      Secondary
      IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution

      Performed under reducing conditions.

      Predicted band size : 27 kDa
      Observed band size : 25 kDa (why is the actual band size different from the predicted?)
    • ab31851 staining rat cochlear nerve cells by ICC/IF. Cells were PFA fixed and permeabilized in 0.1% Triton X-100 prior to blocking in 5% serum for 30 minutes at 25°C. The primary antibody was diluted 1/100 (HBSS with 5% FBS) and incubated with the sample for 18 hours at 4°C. An Alexa Fluor® 568 conjugated goat anti-rabbit antibody, diluted 1/2000, was used as the secondary.

      See Abreview

    • ab31851 staining Myelin Protein Zero in mouse sciatic nerve tissue section by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue from 4% PFA perfused animals underwent overnight fixation in 4% paraformaldehyde, cryoprotected in 30% sucrose and cut using cryostat. The primary antibody was diluted, 1/1000 (PBS + 0.3% Triton X100) and incubated with sample for 18 hours at 20°C. An Alexa Fluor® 488 conjugated goat polyclonal to rabbit IgG was used at 1/1000 dilution, as secondary.

      This image is courtesy of an Abreview submitted by Dr Sophie Pezet.

    • ab31851 staining Myelin Protein Zero in Rat Schwann Cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde and blocked with 5% BSA for 1 hour at room temperature. Samples were incubated with primary antibody (1/250 in 5% NGS/0.3% Triton X-100 in PBS) for 4 hours at 4°C. A Cy2®-conjugated Goat anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody.

      Mpz - Myelin Protein Zero; Mag - Myelin Associated Glycoprotein; Mbp - Myelin Basic Protein; dCKO - double conditional knockout.

      See Abreview

    • ab31851 staining human brainstem sections by IHC-P. The tissue was fixed with formaldehyde and a heat mediated antigen retrival step was performed with citric acid pH 6. Blocking of the sample was done with 1% BSA for 10 minutes at 21°C, followed by staining with ab31851 at 1/200 in TBS/BSA/azide for 16h at 21°C. A biotinylated goat anti-rabbit polyclonal antibody at 1/200 was used as the secondary antibody. The myelinated fibres in the rootlet are positive for this antibody (peripheral nerves).

      See Abreview

    References for Anti-Myelin Protein Zero antibody (ab31851)

    This product has been referenced in:
    • Kosacka J  et al. Increased autophagy in peripheral nerves may protect Wistar Ottawa Karlsburg W rats against neuropathy. Exp Neurol 250:125-35 (2013). WB ; Rat . Read more (PubMed: 24095727) »
    • Makoukji J  et al. Lithium enhances remyelination of peripheral nerves. Proc Natl Acad Sci U S A 109:3973-8 (2012). WB ; Mouse . Read more (PubMed: 22355115) »

    See all 6 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Mouse Tissue lysate - whole (Sciatic Nerve)
    Gel Running Conditions Non-reduced Non-Denaturing (Native)
    Loading amount 30 µg
    Specification Sciatic Nerve
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Aug 05 2016

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunohistochemistry (Frozen sections)
    Sample Mouse Tissue sections (Brain)
    Permeabilization No
    Specification Brain
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
    Fixative Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Mar 04 2016

    Application Western blot
    Sample Rat Tissue lysate - whole (Sciatic nerve)
    Gel Running Conditions Reduced Denaturing (15%)
    Loading amount 20 µg
    Specification Sciatic nerve
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Feb 22 2016

    Application Western blot
    Sample Mouse Tissue lysate - whole (Sciatic nerve)
    Gel Running Conditions Reduced Denaturing (15%)
    Loading amount 20 µg
    Specification Sciatic nerve
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Feb 22 2016

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Mouse Tissue lysate - whole (Sciatic nerve)
    Loading amount 30 µg
    Specification Sciatic nerve
    Gel Running Conditions Reduced Denaturing (15% gel)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Username

    Dr. G.K Sakellariou

    Verified customer

    Submitted May 16 2013

    Thank you for your call yesterday and for your patience while I've looked into your enquiry.
    I've heard back from the lab scientists about ab31851, and we batch test this antibody in Western blot only with rat and mouse sciatic nerve lysate. All o...

    Read More

    Thank you for contacting us.

    Myelin basic protein and myelin protein Zero are the common membrane associated proteins. Please check the datasheets

    ab7349, ab40390, ab62631, ab53294 etc. and ab31851

    ab128836 is an anti myelin ...

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Rat Tissue sections (Spinal cord)
    Specification Spinal cord
    Fixative Formaldehyde
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
    Permeabilization No
    Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
    Username

    Mr. Carl Hobbs

    Verified customer

    Submitted Jun 26 2012

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Mouse Tissue sections (Spinal cord)
    Specification Spinal cord
    Fixative Formaldehyde
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
    Permeabilization No
    Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
    Username

    Mr. Carl Hobbs

    Verified customer

    Submitted Jun 19 2012

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Human Tissue sections (Brainstem)
    Specification Brainstem
    Fixative Formaldehyde
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
    Permeabilization No
    Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
    Username

    Mr. Carl Hobbs

    Verified customer

    Submitted Jun 19 2012

    1-10 of 17 Abreviews or Q&A

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