Anti-Myeloid leukemia factor 1 antibody (ab103099)

Overview

  • Product nameAnti-Myeloid leukemia factor 1 antibody
    See all Myeloid leukemia factor 1 primary antibodies
  • Description
    Rabbit polyclonal to Myeloid leukemia factor 1
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to a region within C terminal amino acids 222-252 of Human Myeloid leukemia factor 1

  • Positive control
    • Mouse heart tissue, Human testis carcinoma

Properties

Applications

Our Abpromise guarantee covers the use of ab103099 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 31 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionInvolved in lineage commitment of primary hemopoietic progenitors by restricting erythroid formation and enhancing myeloid formation. Interferes with erythropoietin-induced erythroid terminal differentiation by preventing cells from exiting the cell cycle through suppression of CDKN1B/p27Kip1 levels. Suppresses RFWD2/COP1 activity via CSN3 which activates p53 and induces cell cycle arrest. Binds DNA and affects the expression of a number of genes so may function as a transcription factor in the nucleus.
  • Tissue specificityMost abundant in testis, ovary, skeletal muscle, heart, kidney and colon. Low expression in spleen, thymus and peripheral blood leukocytes.
  • Involvement in diseaseNote=A chromosomal aberration involving MLF1 is a cause of myelodysplastic syndrome (MDS). Translocation t(3;5)(q25.1;q34) with NPM1/NPM.
  • Sequence similaritiesBelongs to the MLF family.
  • Post-translational
    modifications
    Phosphorylation is required for binding to YWHAZ.
  • Cellular localizationCytoplasm. Nucleus. In non-hematopoietic cells, resides primarily in the cytoplasm with some punctate nuclear localization. Shuttles between the cytoplasm and nucleus. In hematopoietic cells, located preferentially in the nucleus. Found in the nucleolus when fused to NPM.
  • Information by UniProt
  • Database links
  • Alternative names
    • Hls7 antibody
    • MLF1 antibody
    • MLF1_HUMAN antibody
    • Myelodysplasia myeloid leukemia factor 1 antibody
    • Myelodysplasia-myeloid leukemia factor 1 antibody
    • Myeloid leukemia factor 1 antibody
    • myeloid leukemia factor 1 variant 1 antibody
    • myeloid leukemia factor 1 variant 2 antibody
    • myeloid leukemia factor 1 variant 3 antibody
    see all

Anti-Myeloid leukemia factor 1 antibody images

  • Anti-Myeloid leukemia factor 1 antibody (ab103099) at 1/100 dilution + Mouse heart tissue lysate at 35 µg

    Predicted band size : 31 kDa
  • IHC analysis in formalin fixed and paraffin embedded Human testis carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining, using ab103099 at a dilution of 1/50.

References for Anti-Myeloid leukemia factor 1 antibody (ab103099)

ab103099 has not yet been referenced specifically in any publications.

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