Anti-Myeloid leukemia factor 1 antibody (ab70211)


  • Product nameAnti-Myeloid leukemia factor 1 antibody
    See all Myeloid leukemia factor 1 primary antibodies
  • Description
    Rabbit polyclonal to Myeloid leukemia factor 1
  • SpecificityWe have data to indicate that this antibody may not cross react with Mouse. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    A region between residue 200 and the C-terminus (residue 256) of human Myeloid leukemia factor 1.

  • Positive control
    • Whole cell lysate from HeLa cells.



Our Abpromise guarantee covers the use of ab70211 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notesIP: Use at 2 - 5 µg/mg of lysate.
    WB: 1/5000 - 1/15000. Detects a band of approximately 35 kDa (predicted molecular weight: 31 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionInvolved in lineage commitment of primary hemopoietic progenitors by restricting erythroid formation and enhancing myeloid formation. Interferes with erythropoietin-induced erythroid terminal differentiation by preventing cells from exiting the cell cycle through suppression of CDKN1B/p27Kip1 levels. Suppresses RFWD2/COP1 activity via CSN3 which activates p53 and induces cell cycle arrest. Binds DNA and affects the expression of a number of genes so may function as a transcription factor in the nucleus.
    • Tissue specificityMost abundant in testis, ovary, skeletal muscle, heart, kidney and colon. Low expression in spleen, thymus and peripheral blood leukocytes.
    • Involvement in diseaseNote=A chromosomal aberration involving MLF1 is a cause of myelodysplastic syndrome (MDS). Translocation t(3;5)(q25.1;q34) with NPM1/NPM.
    • Sequence similaritiesBelongs to the MLF family.
    • Post-translational
      Phosphorylation is required for binding to YWHAZ.
    • Cellular localizationCytoplasm. Nucleus. In non-hematopoietic cells, resides primarily in the cytoplasm with some punctate nuclear localization. Shuttles between the cytoplasm and nucleus. In hematopoietic cells, located preferentially in the nucleus. Found in the nucleolus when fused to NPM.
    • Information by UniProt
    • Database links
    • Alternative names
      • Hls7 antibody
      • MLF1 antibody
      • MLF1_HUMAN antibody
      • Myelodysplasia myeloid leukemia factor 1 antibody
      • Myelodysplasia-myeloid leukemia factor 1 antibody
      • Myeloid leukemia factor 1 antibody
      • myeloid leukemia factor 1 variant 1 antibody
      • myeloid leukemia factor 1 variant 2 antibody
      • myeloid leukemia factor 1 variant 3 antibody
      see all

    Anti-Myeloid leukemia factor 1 antibody images

    • All lanes : Anti-Myeloid leukemia factor 1 antibody (ab70211) at 0.1 µg/ml

      Lane 1 : Whole cell lysate from HeLa cells at 5 µg
      Lane 2 : Whole cell lysate from HeLa cells at 15 µg
      Lane 3 : Whole cell lysate from HeLa cells at 50 µg

      Predicted band size : 31 kDa
      Observed band size : 35 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 130 kDa,250 kDa,28 kDa. We are unsure as to the identity of these extra bands.
    • Detection of Human Myeloid leukemia factor 1 by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP, 1/4 of IP loaded). Affinity purified rabbit anti-Myeloid leukemia factor 1 antibody (ab70211) was used at 3 µg/mg lysate for IP (Lane 1) and at 0.1 µg/ml for WB detection. Lane 2 represents IP IgG control.

    References for Anti-Myeloid leukemia factor 1 antibody (ab70211)

    ab70211 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab70211.
    Please use the links above to contact us or submit feedback about this product.