Anti-Myeloid Precursor Cells antibody [ER-MP58] (ab63860)

Overview

  • Product nameAnti-Myeloid Precursor Cells antibody [ER-MP58]
    See all Myeloid Precursor Cells primary antibodies
  • Description
    Rat monoclonal [ER-MP58] to Myeloid Precursor Cells
  • Tested applicationsSuitable for: IHC-P, IP, IHC-Fr, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Balb/c macrophage precursor cell hybrids

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityMonoclonal
  • Clone numberER-MP58
  • MyelomaY3/Ag1.2.3
  • IsotypeIgG2a
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab63860 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
IP
IHC-Fr
Flow Cyt
  • Application notesFlow Cyt: Use 10µl for 106 cells.
    IHC-P: Use at an assay dependent dilution (PMID 19720836).
    IHC-Fr: 1/25 - 1/100.
    IP: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceER-MP58 is expressed by all bone marrow derived M-CSF- and GM-CSF-responsive myeloid blood cell precursors. The expression of ER-MP58 remains at a high level throughout the precursor/monocyte stage and is down regulated upon maturation into mature macrophages. The ER-MP58 antigen is used to distinguish between early myeloid committed cells and other haematopoietic progenitors cells in the BM.
    • Cellular localizationCell Membrane

    Anti-Myeloid Precursor Cells antibody [ER-MP58] images

    • ab63860 staining Myeloid Precursor Cells in murine lung tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
      Lungs were excised, fixed in 10% neutral-buffered formalin and embedded in paraffin. Immunohistochemical analysis was performed with heat-induced antigen retrieval. The primary antibody used was ab63860. A biotinylated secondary antibody was used with and DAB detection kit to reveal the positively stained cells with nuclei counterstained with hematoxylin. Scale bar, 200 µm.

    References for Anti-Myeloid Precursor Cells antibody [ER-MP58] (ab63860)

    This product has been referenced in:
    • Karpurapu M  et al. Functional PU.1 in macrophages has a pivotal role in NF-?B activation and neutrophilic lung inflammation during endotoxemia. Blood 118:5255-66 (2011). Flow Cyt ; Mouse . Read more (PubMed: 21937699) »
    • Lu X & Kang Y Chemokine (C-C motif) ligand 2 engages CCR2+ stromal cells of monocytic origin to promote breast cancer metastasis to lung and bone. J Biol Chem 284:29087-96 (2009). IHC-P ; Mouse . Read more (PubMed: 19720836) »

    See all 2 Publications for this product

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    There are currently no Abreviews or Questions for ab63860.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"