The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 0.1µg for 106 cells. ab91356-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
RelevanceER-MP58 is expressed by all bone marrow derived M-CSF- and GM-CSF-responsive myeloid blood cell precursors. The expression of ER-MP58 remains at a high level throughout the precursor/monocyte stage and is down regulated upon maturation into mature macrophages. The ER-MP58 antigen is used to distinguish between early myeloid committed cells and other haematopoietic progenitors cells in the BM.
ab114076, at 0.1 µg/106 cells, staining Rat Myeloid Lineage in Rat (strain: Wistar) bone marrow cells by Flow Cytometry. Isotypic Control: FITC Mouse IgG1 Percentage of cells stained above control: 21.5%
References for Anti-Myeloid Precursor Cells antibody [OX-82] (FITC) (ab114076)
This product has been referenced in:
Crook K & Hunt SV Enrichment of early fetal-liver hemopoietic stem cells of the rat using monoclonal antibodies against the transferrin receptor, Thy-1, and MRC-OX82. Dev Immunol4:235-46 (1996).
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