Anti-Myeloperoxidase antibody [2C7] (ab25989)

Overview

  • Product name
    Anti-Myeloperoxidase antibody [2C7]
    See all Myeloperoxidase primary antibodies
  • Description
    Mouse monoclonal [2C7] to Myeloperoxidase
  • Specificity
    ab25989 recognises myeloperoxidase (MPO).
  • Tested applications
    Suitable for: ICC/IF, IHC-Fr, IHC-P, ELISA, WB, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length native protein (purified) corresponding to Human Myeloperoxidase.

Properties

Applications

Our Abpromise guarantee covers the use of ab25989 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 20818377
IHC-Fr 1/1000 - 1/5000.
IHC-P 1/500 - 1/1000.
ELISA Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Use under non reducing condition. Predicted molecular weight: 84 kDa.
Flow Cyt 1/20 - 1/100.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • Function
    Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity.
  • Involvement in disease
    Defects in MPO are the cause of myeloperoxidase deficiency (MPD) [MIM:254600]. MPD is an autosomal recessive defect that results in disseminated candidiasis.
  • Sequence similarities
    Belongs to the peroxidase family. XPO subfamily.
  • Cellular localization
    Lysosome.
  • Information by UniProt
  • Database links
  • Alternative names
    • 84 kDa myeloperoxidase antibody
    • 89 kDa myeloperoxidase antibody
    • EC 1.11.1.7 antibody
    • EC1.11.2.2 antibody
    • fj80f04 antibody
    • MPO antibody
    • mpx antibody
    • myeloid-specific peroxidase antibody
    • Myeloperoxidase antibody
    • Myeloperoxidase heavy chain antibody
    • Myeloperoxidase light chain antibody
    • PERM_HUMAN antibody
    • wu:fj80f04 antibody
    see all

Anti-Myeloperoxidase antibody [2C7] images

  • Overlay histogram showing HeLa cells stained with ab25989 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab25989, 1/20 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a decreased signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

References for Anti-Myeloperoxidase antibody [2C7] (ab25989)

This product has been referenced in:
  • Wang L  et al. Resistance of hypervirulent Klebsiella pneumoniae to both intracellular and extracellular killing of neutrophils. PLoS One 12:e0173638 (2017). ICC/IF ; Human . Read more (PubMed: 28282434) »
  • Savchenko AS  et al. Neutrophil extracellular traps form predominantly during the organizing stage of human venous thromboembolism development. J Thromb Haemost 12:860-70 (2014). IHC-P ; Human . Read more (PubMed: 24674135) »

See all 14 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (LIVER)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Ph6 (ER1) 20 MINS
Permeabilization
No
Specification
LIVER
Blocking step
H2O2 as blocking agent for 10 minute(s) · Concentration: 3% · Temperature: 21°C
Fixative
Formaldehyde
Username

Mr. Christopher Bagnall

Verified customer

Submitted Nov 22 2016

Thank you for contacting us. Because we carry over 100,000 products, it isn't feasible for us to keep small sample sizes of our products.

We are happy to reassure our customers that all of our products are covered by our Abpromise, which guara...

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DISCOUNT CODE: xxxxxxx Expiration date: 2012-04-20 As discussed I have generated a discount code for ab25989. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for staining ...

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Thank you for contacting us and considering our testing discount with ab25989. As the sequence of human Myeloperoxidase shares 86% homology with the pig protein we would predict that  ab25989 would be able to recognise this protein. I can issue a te...

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Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (ventricular tissue lysates)
Loading amount
50 µg
Specification
ventricular tissue lysates
Gel Running Conditions
Non-reduced Denaturing (4~12% gradient NuPAGE)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Jul 05 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Tissue lysate - whole (human aortic tissue extract)
Loading amount
30 µg
Specification
human aortic tissue extract
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
Username

Dr. Guo-Ping Shi

Verified customer

Submitted Mar 03 2008

Thank you for your enquiry regarding ab25989 and your interest in our products. The immunogen was full-length myeloperoxidase. The antibody recognizes an epitope on the heavy chain, but the exact epitope location has not been mapped.

Ab25989 recognizes an epitope on the heavy chain of myeloperoxidase. The protein is a multimer of 2 heavy chains (55kDa) and two light chains (15kDa), the heavy chains being linked by a disulfide bond. In Western blot under non-reducing conditions, t...

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