The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Flow Cyt: Use 10 µl of undiluted antibody to label 106 cells in 100 µl. This antibody may be used for the flow cytometric detection of myeloid cells following membrane permeablisation.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity.
Involvement in disease
Defects in MPO are the cause of myeloperoxidase deficiency (MPD) [MIM:254600]. MPD is an autosomal recessive defect that results in disseminated candidiasis.
Patschan S et al. Impairment and Differential Expression of PR3 and MPO on Peripheral Myelomonocytic Cells with Endothelial Properties in Granulomatosis with Polyangiitis. Int J Nephrol2012:715049 (2012).
Read more (PubMed: 22792461) »