Anti-Myeloperoxidase antibody [2D4] (ab90810)

Overview

  • Product name
    Anti-Myeloperoxidase antibody [2D4]
    See all Myeloperoxidase primary antibodies
  • Description
    Mouse monoclonal [2D4] to Myeloperoxidase
  • Tested applications
    Suitable for: IHC-Fr, Flow Cyt, ICC/IF, ELISAmore details
    Unsuitable for: IHC-P or WB
  • Species reactivity
    Reacts with: Mouse, Rat
  • Immunogen

    Antigen used is purified natural MPO.

Properties

Applications

Our Abpromise guarantee covers the use of ab90810 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/50.
Flow Cyt 1/50. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC/IF 1/50.
ELISA Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for IHC-P or WB.
  • Target

    • Function
      Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity.
    • Involvement in disease
      Defects in MPO are the cause of myeloperoxidase deficiency (MPD) [MIM:254600]. MPD is an autosomal recessive defect that results in disseminated candidiasis.
    • Sequence similarities
      Belongs to the peroxidase family. XPO subfamily.
    • Cellular localization
      Lysosome.
    • Information by UniProt
    • Database links
    • Alternative names
      • 84 kDa myeloperoxidase antibody
      • 89 kDa myeloperoxidase antibody
      • EC 1.11.1.7 antibody
      • EC1.11.2.2 antibody
      • fj80f04 antibody
      • MPO antibody
      • mpx antibody
      • myeloid-specific peroxidase antibody
      • Myeloperoxidase antibody
      • Myeloperoxidase heavy chain antibody
      • Myeloperoxidase light chain antibody
      • PERM_HUMAN antibody
      • wu:fj80f04 antibody
      see all

    Anti-Myeloperoxidase antibody [2D4] images

    • ab90810 at 1/50 dilution staining Myeloperoxidase in rat white blood cells by Immunocytochemistry/ Immunofluorescence. Image shows green staining with ab90810 in neutrophils and monocytes.

    References for Anti-Myeloperoxidase antibody [2D4] (ab90810)

    This product has been referenced in:
    • Abdollahi M  et al. Insulin treatment prevents wounding associated changes in tissue and circulating neutrophil MMP-9 and NGAL in diabetic rats. PLoS One 12:e0170951 (2017). ICC/IF ; Rat . Read more (PubMed: 28182694) »
    • Jie H  et al. Necrostatin-1 enhances the resolution of inflammation by specifically inducing neutrophil apoptosis. Oncotarget 7:19367-81 (2016). IHC . Read more (PubMed: 27027357) »

    See all 3 Publications for this product

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Sheep Cell (Lung)
    Specification
    Lung
    Fixative
    Formaldehyde
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
    Username

    Abcam user community

    Verified customer

    Submitted May 13 2013

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    Myeloperoxidase is known to be expressed in a subset of lymphoid cells. Tissues known to express this protein include spleen, lymph node and heatopoietic cells of bone marrow.Any of these...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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