The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50. ab91356-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
Use at an assay dependent concentration.
Application notesIs unsuitable for IHC-P or WB.
FunctionPart of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity.
Involvement in diseaseDefects in MPO are the cause of myeloperoxidase deficiency (MPD) [MIM:254600]. MPD is an autosomal recessive defect that results in disseminated candidiasis.
Sequence similaritiesBelongs to the peroxidase family. XPO subfamily.
Immunohistochemistry (Frozen sections) - Anti-Myeloperoxidase antibody [2D4] (FITC) (ab90812)Image from El-Zaatari, Mohamad et al., PLoS ONE 8.3 (2013): e58935. doi: 10.1371/journal.pone.0058935.Fig S3C.
Immunohistochemistry (Frozen sections) analysis of mouse stomach tissue labeling Myeloperoxidase with ab90812 (green). 8 µm sections were fixed in 4% paraformaldehyde for 10 min, washed in PBS twice, and then blocked with 20% donkey serum in PBS. β-gal (red), and DAPI (blue).
References for Anti-Myeloperoxidase antibody [2D4] (FITC) (ab90812)
This product has been referenced in:
El-Zaatari M et al. Tryptophan catabolism restricts IFN-?-expressing neutrophils and Clostridium difficile immunopathology. J Immunol193:807-16 (2014).
Read more (PubMed: 24935925) »
El-Zaatari M et al. Gli1 deletion prevents Helicobacter-induced gastric metaplasia and expansion of myeloid cell subsets. PLoS One8:e58935 (2013).
Read more (PubMed: 23520544) »