Anti-Myeloperoxidase antibody [8F4] (ab16886)

Overview

  • Product name
    Anti-Myeloperoxidase antibody [8F4]
    See all Myeloperoxidase primary antibodies
  • Description
    Mouse monoclonal [8F4] to Myeloperoxidase
  • Tested applications
    Suitable for: IHC-Fr, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat
  • Immunogen

    Purified mouse Myeloperoxidase from WEHI-3 cells.

  • Positive control
    • Neutrophils isolated from digested infarcts

Properties

Applications

Our Abpromise guarantee covers the use of ab16886 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/10.
Flow Cyt 1/10. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • Function
    Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity.
  • Involvement in disease
    Defects in MPO are the cause of myeloperoxidase deficiency (MPD) [MIM:254600]. MPD is an autosomal recessive defect that results in disseminated candidiasis.
  • Sequence similarities
    Belongs to the peroxidase family. XPO subfamily.
  • Cellular localization
    Lysosome.
  • Information by UniProt
  • Database links
  • Alternative names
    • 84 kDa myeloperoxidase antibody
    • 89 kDa myeloperoxidase antibody
    • EC 1.11.1.7 antibody
    • EC1.11.2.2 antibody
    • fj80f04 antibody
    • MPO antibody
    • mpx antibody
    • myeloid-specific peroxidase antibody
    • Myeloperoxidase antibody
    • Myeloperoxidase heavy chain antibody
    • Myeloperoxidase light chain antibody
    • PERM_HUMAN antibody
    • wu:fj80f04 antibody
    see all

Anti-Myeloperoxidase antibody [8F4] images

  • Detection of MPO in Wehi3BD+ cells. Red, black and blue line represent the isotype control, cells only and ab16886 at 10 μg/ml, respectively.

References for Anti-Myeloperoxidase antibody [8F4] (ab16886)

This product has been referenced in:
  • Lu SM  et al. HIV-1 Tat-Induced Microgliosis and Synaptic Damage via Interactions between Peripheral and Central Myeloid Cells. PLoS One 6:e23915 (2011). IHC-FoFr ; Mouse . Read more (PubMed: 21912650) »
  • Salguero Palacios R  et al. Activation of hepatic stellate cells is associated with cytokine expression in thioacetamide-induced hepatic fibrosis in mice. Lab Invest 88:1192-203 (2008). IHC-Fr ; Mouse . Read more (PubMed: 18794850) »

See all 2 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Rat Cell lysate - whole cell (Rat Neutrophils and Human MPO)
Loading amount
6 µg
Specification
Rat Neutrophils and Human MPO
Gel Running Conditions
Reduced Denaturing (7.5)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5.0% · Temperature: 23°C
Username

Mrs. Donna Flanagan

Verified customer

Submitted Mar 13 2009

As far as we know, the epitope has not been mapped. Please contact us again if you have any additional questions.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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