Overview

  • Product nameAnti-Myeloperoxidase antibody [EPR4792]
    See all Myeloperoxidase primary antibodies
  • Description
    Rabbit monoclonal [EPR4792] to Myeloperoxidase
  • Specificityab109116 recognizes Myeloperoxidase (85-90 kDa) and its light chain (15 kDa).
  • Tested applicationsSuitable for: ICC/IF, WB, IHC-P, ICCmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide, corresponding to residues in Human Myeloperoxidase.

  • Positive control
    • WB: HL-60 cell lysate IHC-P: Human colon, lung and spleen tissues
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Applications

Our Abpromise guarantee covers the use of ab109116 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 24691127
WB 1/1000 - 1/10000. Predicted molecular weight: 84 kDa.
IHC-P 1/250 - 1/500. Antigen retrieval is recommended.
ICC 1/250 - 1/500.
  • Application notesIs unsuitable for Flow Cyt or IP.
  • Target

    • FunctionPart of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity.
    • Involvement in diseaseDefects in MPO are the cause of myeloperoxidase deficiency (MPD) [MIM:254600]. MPD is an autosomal recessive defect that results in disseminated candidiasis.
    • Sequence similaritiesBelongs to the peroxidase family. XPO subfamily.
    • Cellular localizationLysosome.
    • Information by UniProt
    • Database links
    • Alternative names
      • 84 kDa myeloperoxidase antibody
      • 89 kDa myeloperoxidase antibody
      • EC 1.11.1.7 antibody
      • EC1.11.2.2 antibody
      • fj80f04 antibody
      • MPO antibody
      • mpx antibody
      • myeloid-specific peroxidase antibody
      • Myeloperoxidase antibody
      • Myeloperoxidase heavy chain antibody
      • Myeloperoxidase light chain antibody
      • PERM_HUMAN antibody
      • wu:fj80f04 antibody
      see all

    Anti-Myeloperoxidase antibody [EPR4792] images

    • Anti-Myeloperoxidase antibody [EPR4792] (ab109116) at 1/1000 dilution + HL-60 cell lysate at 10 µg

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 84 kDa
      Observed band size : 85-90 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 15 kDa (possible cleavage fragment).
    • ab109116, at a 1/250 dilution, staining Myeloperoxidase in paraffin embedded Human colon tissue by Immunohistochemistry.
    • ab109116, at a 1/250 dilution, staining Myeloperoxidase in paraffin embedded Human lung tissue by Immunohistochemistry.
    • ab109116, at a 1/250 dilution, staining Myeloperoxidase in paraffin embedded Human spleen tissue by Immunohistochemistry.
    • ab109116 showing positive staining in Normal liver tissue.

    • ab109116 showing positive staining in Bone marrow tissue.

    • ab109116 showing negative staining in Skeletal muscle tissue.

    References for Anti-Myeloperoxidase antibody [EPR4792] (ab109116)

    This product has been referenced in:
    • Baek JH  et al. Extracellular Hb enhances cardiac toxicity in endotoxemic guinea pigs: protective role of haptoglobin. Toxins (Basel) 6:1244-59 (2014). WB, ICC/IF ; Guinea pig . Read more (PubMed: 24691127) »

    See 1 Publication for this product

    Product Wall

    There are currently no Abreviews or Questions for ab109116.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"