Overview

  • Product nameAnti-Myf5 antibody
    See all Myf5 primary antibodies
  • Description
    Rabbit polyclonal to Myf5
  • Tested applicationsSuitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Macaque Monkey, Orangutan
  • Immunogen

    Synthetic peptide within Human Myf5 aa 200-300. The exact sequence is proprietary.

  • Positive control
    • This antibody gave a positive signal in Human Fetal Skeletal Muscle and Mouse E10 Whole Embryo tissue lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab125301 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 24699686
WB Use a concentration of 1 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa).

Target

Anti-Myf5 antibody images

  • All lanes : Anti-Myf5 antibody (ab125301) at 1 µg/ml

    Lane 1 : Skeletal Muscle (Human) Tissue Lysate - Fetal Tissue
    Lane 2 : Mouse Embryo E10 Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 28 kDa
    Observed band size : 28 kDa
    Additional bands at : 46 kDa,52 kDa,98 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes

References for Anti-Myf5 antibody (ab125301)

This product has been referenced in:
  • Ren Y  et al. Potential of adipose-derived mesenchymal stem cells and skeletal muscle-derived satellite cells for somatic cell nuclear transfer mediated transgenesis in Arbas Cashmere goats. PLoS One 9:e93583 (2014). ICC/IF ; Goat . Read more (PubMed: 24699686) »

See 1 Publication for this product

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"