Mouse, Human Predicted to work with:
Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Macaque monkey, Orangutan
Synthetic peptide within Human Myf5 aa 200-300. The exact sequence is proprietary.
This antibody gave a positive signal in Human Fetal Skeletal Muscle and Mouse E10 Whole Embryo tissue lysates.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40 Preservative: 0.02% Sodium azide Constituent: PBS Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
All lanes : Anti-Myf5 antibody (ab125301) at 1 µg/ml
Lane 1 : Skeletal Muscle (Human) Tissue Lysate - Fetal Tissue Lane 2 : Mouse Embryo E10 Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution Developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 28 kDa Observed band size : 28 kDa Additional bands at : 46 kDa,52 kDa,98 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 20 minutes
References for Anti-Myf5 antibody (ab125301)
This product has been referenced in:
Ren Y et al. Potential of adipose-derived mesenchymal stem cells and skeletal muscle-derived satellite cells for somatic cell nuclear transfer mediated transgenesis in Arbas Cashmere goats. PLoS One9:e93583 (2014).
Read more (PubMed: 24699686) »