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Rabbit polyclonal to Myf5
Detects endogenous levels of total Myf5 protein. Cross-reactivity with Myf5, MyoD and Myf6 (greater than 85% sequence homology).
HumanPredicted to work with:
Synthesized peptide derived from an internal sequence of human Myf5.
- Extracts from HepG2 cells
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
Concentration information loading...
Immunogen affinity purified
Affinity purified from rabbit antiserum by affinity chromatography, using
epitope specific immunogen.
Our Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/500 - 1/1000. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa).
Involved in muscle differentiation (myogenic factor). Induces fibroblasts to differentiate into myoblasts. Probable sequence specific DNA-binding protein.
Contains 1 basic helix-loop-helix (bHLH) domain.
Information by UniProt
- bHLHc2 antibody
- Class C basic helix loop helix protein 2 antibody
- Class C basic helix-loop-helix protein 2 antibody
Western blot - Myf5 antibody (ab69997)
All lanes : Anti-Myf5 antibody (ab69997) at 1/500 dilution
Lane 1 : Extracts from HepG2 cells, minus immunising peptide
Lane 2 : Extracts from HepG2 cells, plus immunising peptide at 5µg
Lysates/proteins at 5 µg per lane.
Predicted band size : 28 kDa
Observed band size : 28 kDa
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