Overview

  • Product nameAnti-Myf5 antibody
    See all Myf5 primary antibodies
  • Description
    Rabbit polyclonal to Myf5
  • SpecificityDetects endogenous levels of total Myf5 protein. Cross-reactivity with Myf5, MyoD and Myf6 (greater than 85% sequence homology).
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthesized peptide derived from an internal sequence of human Myf5.

  • Positive control
    • Extracts from HepG2 cells

Properties

Applications

Our Abpromise guarantee covers the use of ab69997 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa).
ELISA 1/40000.

Target

Anti-Myf5 antibody images

  • All lanes : Anti-Myf5 antibody (ab69997) at 1/500 dilution

    Lane 1 : Extracts from HepG2 cells, minus immunising peptide
    Lane 2 : Extracts from HepG2 cells, plus immunising peptide at 5µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 28 kDa
    Observed band size : 28 kDa

References for Anti-Myf5 antibody (ab69997)

This product has been referenced in:
  • Bareja A  et al. Human and mouse skeletal muscle stem cells: convergent and divergent mechanisms of myogenesis. PLoS One 9:e90398 (2014). ICC ; Mouse, Human . Read more (PubMed: 24587351) »
  • Koch AJ & Holaska JM Loss of Emerin Alters Myogenic Signaling and miRNA Expression in Mouse Myogenic Progenitors. PLoS One 7:e37262 (2012). WB . Read more (PubMed: 22606356) »

See all 3 Publications for this product

Product Wall

Application Western blot
Sample Rabbit Tissue lysate - whole (Muscle)
Gel Running Conditions Reduced Denaturing (15%)
Loading amount 35 µg
Specification Muscle
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
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Submitted Mar 06 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"