Recombinant Anti-Myf5 antibody [EPR4899] (ab125078)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4899] to Myf5
- Suitable for: WB, IP
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
-
Product name
Anti-Myf5 antibody [EPR4899]
See all Myf5 primary antibodies -
Description
Rabbit monoclonal [EPR4899] to Myf5 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details
Unsuitable for: ICC/IF -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Pig -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- WB: Human skeletal muscle, rat muscle, mouse muscle and rat heart tissue lysate.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR4899 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab125078 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/10000 - 1/50000. Detects a band of approximately 37 kDa (predicted molecular weight: 28 kDa).
|
|
IP |
1/20.
|
Notes |
---|
WB
1/10000 - 1/50000. Detects a band of approximately 37 kDa (predicted molecular weight: 28 kDa). |
IP
1/20. |
Target
-
Function
Involved in muscle differentiation (myogenic factor). Induces fibroblasts to differentiate into myoblasts. Probable sequence specific DNA-binding protein. -
Sequence similarities
Contains 1 basic helix-loop-helix (bHLH) domain. -
Cellular localization
Nucleus. - Information by UniProt
-
Database links
- Entrez Gene: 4617 Human
- Entrez Gene: 17877 Mouse
- Entrez Gene: 407603 Pig
- Entrez Gene: 299766 Rat
- Omim: 159990 Human
- SwissProt: P13349 Human
- SwissProt: P24699 Mouse
- Unigene: 178023 Human
see all -
Alternative names
- bHLHc2 antibody
- Class C basic helix loop helix protein 2 antibody
- Class C basic helix-loop-helix protein 2 antibody
see all
Images
-
Anti-Myf5 antibody [EPR4899] (ab125078) at 1/50000 dilution (purified) + Human skeletal muscle tissue lysate at 10 µg
Secondary
HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 28 kDa
Observed band size: 39 kDa why is the actual band size different from the predicted?
Blocking and dilution buffer: 5% NFDM/TBST -
All lanes : Anti-Myf5 antibody [EPR4899] (ab125078) at 1/50000 dilution (purified)
Lane 1 : Rat muscle tissue lysate
Lane 2 : Mouse muscle tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 28 kDa
Observed band size: 39 kDa why is the actual band size different from the predicted?
Blocking and dilution buffer: 5% NFDM/TBST -
ab125078 (Purified) at 1/20 dilution (20 µg/ml) immunoprecipitating Myf5 in Human skeletal muscle lysate.
Lane 1 (input): Human skeletal muscle
Lane 2 (+): 125078 & Human skeletal muscle lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab125078 in human skeletal muscle lysate
For western blotting, ab125078 at 1/1000 and VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/1000 dilution.
Blocking and diluting buffer: 5% NFDM /TBST. -
All lanes : Anti-Myf5 antibody [EPR4899] (ab125078) at 1/10000 dilution (unpurified)
Lane 1 : Human skeletal muscle tissue lysate
Lane 2 : Rat muscle tissue lysate
Lane 3 : Mouse muscle tissue lysate
Lane 4 : Rat heart tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 28 kDa
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (11)
ab125078 has been referenced in 11 publications.
- Yang C et al. Circular RNA RBPMS inhibits bladder cancer progression via miR-330-3p/RAI2 regulation. Mol Ther Nucleic Acids 23:872-886 (2021). PubMed: 33614236
- Zheng Y et al. Integrated analysis of long non-coding RNAs (lncRNAs) and mRNA expression profiles identifies lncRNA PRKG1-AS1 playing important roles in skeletal muscle aging. Aging (Albany NY) 13:15044-15060 (2021). PubMed: 34051073
- Chaudhary P et al. High altitude mediated skeletal muscle atrophy: Protective role of curcumin. Biochimie 156:138-147 (2019). PubMed: 30347230
- Stewart E et al. Identification of Therapeutic Targets in Rhabdomyosarcoma through Integrated Genomic, Epigenomic, and Proteomic Analyses. Cancer Cell 34:411-426.e19 (2018). PubMed: 30146332
- Boularaoui SM et al. Efficient transdifferentiation of human dermal fibroblasts into skeletal muscle. J Tissue Eng Regen Med 12:e918-e936 (2018). PubMed: 28101909