Overview

  • Product nameAnti-Myf5 (phospho S49) antibody
    See all Myf5 primary antibodies
  • Description
    Rabbit polyclonal to Myf5 (phospho S49)
  • Specificityab63563 detects endogenous levels of Myf5 only when phosphorylated at serine 49.
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthesized phosphopeptide derived from human Myf5 around the phosphorylation site of serine 49 (Q-G-SP-D-E).

  • Positive control
    • HeLa nuclear lysate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab63563 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 34 kDa (predicted molecular weight: 28 kDa).
ELISA 1/5000.

Target

Anti-Myf5 (phospho S49) antibody images

  • All lanes : Anti-Myf5 (phospho S49) antibody (ab63563) at 1/500 dilution

    Lane 1 : Extracts from HeLa cells (5-30µg) minus immunising peptide
    Lane 2 : Extracts from HeLa cells (5-30µg) plus immunising peptide (5-10µg)


    Predicted band size : 28 kDa
    Observed band size : 34 kDa (why is the actual band size different from the predicted?)

References for Anti-Myf5 (phospho S49) antibody (ab63563)

ab63563 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"