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Storage instructions Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
Concentration information loading...
Purity Immunogen affinity purified
Purification notes ab63563 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 34 kDa (predicted molecular weight: 28 kDa).
Function Involved in muscle differentiation (myogenic factor). Induces fibroblasts to differentiate into myoblasts. Probable sequence specific DNA-binding protein.
Sequence similarities Contains 1 basic helix-loop-helix (bHLH) domain.
Cellular localization Nucleus.
Information by UniProt
Class C basic helix loop helix protein 2 antibody
Class C basic helix-loop-helix protein 2 antibody
Anti-Myf5 (phospho S49) antibody images
Western blot - Myf5 (phospho S49) antibody (ab63563)
All lanes : Anti-Myf5 (phospho S49) antibody (ab63563) at 1/500 dilution Lane 1 : Extracts from HeLa cells (5-30µg) minus immunising peptide Lane 2 : Extracts from HeLa cells (5-30µg) plus immunising peptide (5-10µg) Predicted band size : 28 kDa Observed band size : 34 kDa ( why is the actual band size different from the predicted?)
References for Anti-Myf5 (phospho S49) antibody (ab63563)
has not yet been referenced specifically in any publications.
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