Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab82842 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
WB
  • Application notesELISA: Use at an assay dependent dilution.
    Antibody specificity was verified by direct ELISA against the 3 immunogen peptides. A minimum titer of 1/17000 is determined for one of the three peptides.
    WB: 1/1000. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionInvolved in muscle differentiation (myogenic factor). Induces fibroblasts to differentiate into myoblasts. Probable sequence specific DNA-binding protein.
    • Tissue specificitySkeletal muscle.
    • Involvement in diseaseDefects in MYF6 may be a cause of centronuclear myopathy autosomal dominant (ADCNM) [MIM:160150]; also known as autosomal dominant myotubular myopathy. Centronuclear myopathies are congenital muscle disorders characterized by progressive muscular weakness and wasting involving mainly limb girdle, trunk, and neck muscles. It may also affect distal muscles. Weakness may be present during childhood or adolescence or may not become evident until the third decade of life. Ptosis is a frequent clinical feature. The most prominent histopathologic features include high frequency of centrally located nuclei in muscle fibers not secondary to regeneration, radial arrangement of sarcoplasmic strands around the central nuclei, and predominance and hypotrophy of type 1 fibers.
    • Sequence similaritiesContains 1 basic helix-loop-helix (bHLH) domain.
    • Cellular localizationNucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • bHLHc4 antibody
      • Class C basic helix-loop-helix protein 4 antibody
      • CNM3 antibody
      • HERCULIN antibody
      • MRF4 antibody
      • Muscle-regulatory factor 4 antibody
      • Muscle-specific regulatory factor 4 antibody
      • Myf-6 antibody
      • Myf6 antibody
      • MYF6_HUMAN antibody
      • myogenic factor 6 (herculin) antibody
      • Myogenic factor 6 antibody
      see all

    Anti-MYF6 antibody images

    • All lanes : Anti-MYF6 antibody (ab82842) at 1/1000 dilution

      Lane 1 : uninduced culture of E.coli (one shot Top10 competent cells)
      Lane 2 : induced culture of E.coli (one shot Top10 competent cells)

      Secondary
      anti-Rabbit IgG HRP at 1/1000 dilution

      Predicted band size : 41 kDa
      Observed band size : 41 kDa
      Additional bands at : 60 kDa,95 kDa. We are unsure as to the identity of these extra bands.

    References for Anti-MYF6 antibody (ab82842)

    ab82842 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"