Overview

  • Product name
  • Description
    Rabbit polyclonal to MYH
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human MYH (C terminal). (C terminal 300 amino acids with tag; BC003178).
    Database link: Q9UIF7-3

  • Positive control
    • Human liver cancer and stomach cancer tissue lysates; Human lung cancer tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.30
    Preservative: 0.05% Sodium azide
    Constituents: 50% Glycerol, 49% PBS

    Note: PBS without Mg2+ and Ca2+.
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab182759 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 59 kDa.
IHC-P 1/50 - 1/200.

Target

  • Function
    Involved in oxidative DNA damage repair. Initiates repair of A*oxoG to C*G by removing the inappropriately paired adenine base from the DNA backbone. Possesses both adenine and 2-OH-A DNA glycosylase activities.
  • Involvement in disease
    Defects in MUTYH are a cause of familial adenomatous polyposis type 2 (FAP2) [MIM:608456]. A condition characterized by the development of multiple colorectal adenomatous polyps, benign neoplasms derived from glandular epithelium. Some affected individuals may develop colorectal carcinoma.
    Defects in MUTYH are a cause of gastric cancer (GASC) [MIM:613659]. A malignant disease which starts in the stomach, can spread to the esophagus or the small intestine, and can extend through the stomach wall to nearby lymph nodes and organs. It also can metastasize to other parts of the body. The term gastric cancer or gastric carcinoma refers to adenocarcinoma of the stomach that accounts for most of all gastric malignant tumors. Two main histologic types are recognized, diffuse type and intestinal type carcinomas. Diffuse tumors are poorly differentiated infiltrating lesions resulting in thickening of the stomach. In contrast, intestinal tumors are usually exophytic, often ulcerating, and associated with intestinal metaplasia of the stomach, most often observed in sporadic disease.
  • Sequence similarities
    Belongs to the Nth/MutY family.
    Contains 1 nudix hydrolase domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • A/G specific adenine DNA glycosylase antibody
    • A/G-specific adenine DNA glycosylase antibody
    • Adenine DNA glycosylase antibody
    • CYP2C antibody
    • hMYH antibody
    • MGC4416 antibody
    • MutY (E. coli) homolog antibody
    • MutY homolog (E. coli) antibody
    • MutY homolog antibody
    • MutY, E. coli, homolog of antibody
    • Mutyh antibody
    • MUTYH_HUMAN antibody
    • MYH antibody
    • rMYH antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded Human lung cancer tissue labeling MYH with ab182759 at 1/60 dilution (left panel). Right panel is treated with the immunogen.

  • All lanes : Anti-MYH antibody (ab182759) at 1/1000 dilution

    Lane 1 : Human liver cancer tissue lysate
    Lane 2 : Human stomach cancer tissue lysate

    Lysates/proteins at 40 µg per lane.

    Secondary
    All lanes : Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution

    Developed using the ECL technique.

    Predicted band size: 59 kDa


    Exposure time: 1 minute


    Gel: 8% SDS-PAGE

References

ab182759 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (lung carcinoma and glioblastoma)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate
Specification
lung carcinoma and glioblastoma
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 22°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted Oct 14 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up