Overview

  • Product name
    Anti-MYH4 antibody
  • Description
    Rabbit polyclonal to MYH4
  • Tested applications
    Suitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide derived from Human MYH4 at C-terminal

  • Positive control
    • Human skeletal muscle tissue

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer
    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 50% Glycerol, 49% PBS, 0.88% Sodium chloride
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab111442 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Muscle contraction.
  • Sequence similarities
    Contains 1 IQ domain.
    Contains 1 myosin head-like domain.
  • Domain
    The rodlike tail sequence is highly repetitive, showing cycles of a 28-residue repeat pattern composed of 4 heptapeptides, characteristic for alpha-helical coiled coils.
    Each myosin heavy chain can be split into 1 light meromyosin (LMM) and 1 heavy meromyosin (HMM). It can later be split further into 2 globular subfragments (S1) and 1 rod-shaped subfragment (S2).
  • Cellular localization
    Cytoplasm > myofibril. Thick filaments of the myofibrils.
  • Information by UniProt
  • Database links
  • Alternative names
    • fetal antibody
    • MYH2B antibody
    • Myh4 antibody
    • MYH4_HUMAN antibody
    • MyHC-2b antibody
    • MyHC-IIb antibody
    • Myosin heavy chain 2b antibody
    • Myosin heavy chain 4 antibody
    • Myosin heavy chain 4 skeletal muscle antibody
    • Myosin heavy chain antibody
    • Myosin heavy chain IIb antibody
    • Myosin heavy chain skeletal muscle fetal antibody
    • Myosin-4 antibody
    • skeletal muscle antibody
    see all

Images

  • ab111442, at 1/50 dilution, staining MYH4 in paraffin-embedded Human skeletal muscle tissue by Immunohistochemistry, in the presence (right panel) or absence (left panel) of immunising peptide.

References

ab111442 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Pig Tissue lysate - whole (Muscle)
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
40 µg
Specification
Muscle
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 500µg/mL · Temperature: 34°C
Username

Md Shamimul Hasan

Verified customer

Submitted Jul 20 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up