Overview

  • Product nameAnti-MYH4 antibody
  • Description
    Rabbit polyclonal to MYH4
  • Tested applicationsSuitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide derived from Human MYH4 at C-terminal

  • Positive control
    • Human skeletal muscle tissue

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage bufferpH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 50% Glycerol, 49% PBS, 0.88% Sodium chloride
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab111442 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionMuscle contraction.
  • Sequence similaritiesContains 1 IQ domain.
    Contains 1 myosin head-like domain.
  • DomainThe rodlike tail sequence is highly repetitive, showing cycles of a 28-residue repeat pattern composed of 4 heptapeptides, characteristic for alpha-helical coiled coils.
    Each myosin heavy chain can be split into 1 light meromyosin (LMM) and 1 heavy meromyosin (HMM). It can later be split further into 2 globular subfragments (S1) and 1 rod-shaped subfragment (S2).
  • Cellular localizationCytoplasm > myofibril. Thick filaments of the myofibrils.
  • Information by UniProt
  • Database links
  • Alternative names
    • fetal antibody
    • MYH2B antibody
    • Myh4 antibody
    • MYH4_HUMAN antibody
    • MyHC-2b antibody
    • MyHC-IIb antibody
    • Myosin heavy chain 2b antibody
    • Myosin heavy chain 4 antibody
    • Myosin heavy chain 4 skeletal muscle antibody
    • Myosin heavy chain antibody
    • Myosin heavy chain IIb antibody
    • Myosin heavy chain skeletal muscle fetal antibody
    • Myosin-4 antibody
    • skeletal muscle antibody
    see all

Anti-MYH4 antibody images

  • ab111442, at 1/50 dilution, staining MYH4 in paraffin-embedded Human skeletal muscle tissue by Immunohistochemistry, in the presence (right panel) or absence (left panel) of immunising peptide.

References for Anti-MYH4 antibody (ab111442)

ab111442 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Pig Tissue lysate - whole (Muscle)
Gel Running Conditions Reduced Denaturing (4-12%)
Loading amount 40 µg
Specification Muscle
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 500µg/mL · Temperature: 34°C
Username

Mr. Md Shamimul Hasan

Verified customer

Submitted Jul 20 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"