Recombinant
RabMAb

Anti-MYH7B antibody [EPR12290] (ab172967)

Overview

  • Product name
    Anti-MYH7B antibody [EPR12290]
  • Description
    Rabbit monoclonal [EPR12290] to MYH7B
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    within Human MYH7B. The exact sequence is proprietary.
    Database link: A7E2Y1

  • Positive control
    • WB: Human fetal muscle, fetal heart and skeletal muscle lysates. IHC-P: Human skeletal muscle, cardiac muscle, pancreas and heart tissues. ICC/IF: Rat glial tumor cell line.
  • General notes

    The mouse and rat recommendation is based on the WB results. This antibody may not be suitable for IHC with mouse or rat samples.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 0.05% BSA, 40% Glycerol
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    EPR12290
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab172967 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 221 kDa.
IHC-P 1/1000.

See IHC antigen retrieval protocols.

For unpurified format use at 1/250 - 1/500. 

Target

  • Function
    Involved in muscle contraction.
  • Tissue specificity
    Expressed in heart, skeletal muscle, testis, and all specific brain regions examined. Slightly lower expression was detected in ovary and kidney, and intermediate expression was detected in lung, pancreas, spleen and liver.
  • Sequence similarities
    Contains 1 IQ domain.
    Contains 1 myosin head-like domain.
  • Developmental stage
    Found in fetal liver and brain.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Antigen MLAA-21 antibody
    • cardiac muscle beta isoform antibody
    • Myh7b antibody
    • MYH7B_HUMAN antibody
    • Myosin cardiac muscle beta chain antibody
    • Myosin heavy chain 7B antibody
    • Myosin-7B antibody
    • Slow A MYH14 antibody
    see all

Images

  • Immunohistochemical analysis of paraffin embedded Human heart tissue labeling MYH7B with ab172967 at a 1/250 dilution.

  • Anti-MYH7B antibody [EPR12290] (ab172967) at 0.05 µg/ml (Purified) + Human fetal muscle lysate at 20 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

    Predicted band size: 221 kDa
    Observed band size: 221 kDa



    Blocking and diluting buffer: 5% NFDM/TBST

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human cardiac muscle tissue sections labeling MYH7B with Purified ab172967 at 1:1000 dilution (1.03 μg/ml). Heat mediated antigen retrieval was performed using Perform heat mediated antigen retrieval using EDTA Buffer, PH9. Tissue was counterstained with Hematoxylin. ab97051 Goat Anti-Rabbit IgG H&L (HRP) secondary antibody was used at 1:500 dilution. PBS instead of the primary antibody was used as the negative control.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human skeletal muscle tissue sections labeling MYH7B with Purified ab172967 at 1:1000 dilution (1.03 μg/ml). Heat mediated antigen retrieval was performed using Perform heat mediated antigen retrieval using EDTA Buffer, PH9. Tissue was counterstained with Hematoxylin. ab97051 Goat Anti-Rabbit IgG H&L (HRP) secondary antibody was used at 1:500 dilution. PBS instead of the primary antibody was used as the negative control.

  • All lanes : Anti-MYH7B antibody [EPR12290] (ab172967) at 0.1 µg/ml (Purified)

    Lane 1 : Mouse heart lysate
    Lane 2 : Rat heart lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

    Predicted band size: 221 kDa
    Observed band size: 221 kDa



    Blocking and diluting buffer: 5% NFDM/TBST

  • ab172967 showing +ve staining in Human normal pancreas tissue.

  • Immunohistochemical analysis of paraffin embedded Human skeletal muscle tissue labeling MYH7B with ab172967 at a 1/250 dilution.

  • ab172967 showing -ve staining in Human normal liver tissue.
  • ab172967 showing -ve staining in Human normal breast tissue.
  • All lanes : Anti-MYH7B antibody [EPR12290] (ab172967) at 1/1000 dilution (unpurified)

    Lane 1 : Human fetal muscle lysates
    Lane 2 : Human fetal heart lysates
    Lane 3 : Human skeletal muscle lysates

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 221 kDa

References

This product has been referenced in:
  • Nandi SS & Mishra PK H2S and homocysteine control a novel feedback regulation of cystathionine beta synthase and cystathionine gamma lyase in cardiomyocytes. Sci Rep 7:3639 (2017). WB ; Mouse . Read more (PubMed: 28623294) »
  • Zeng X  et al. Acylated and unacylated ghrelin inhibit atrophy in myotubes co-cultured with colon carcinoma cells. Oncotarget 8:72872-72885 (2017). Read more (PubMed: 29069832) »

See all 6 Publications for this product

Customer reviews and Q&As

Application
Immunohistochemistry (Frozen sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Sample
Human Tissue sections (heart)
Specification
heart
Permeabilization
Yes - Triton x-100, 0.01%
Fixative
Paraformaldehyde
Username

Dr. Shyam Sundar Nandi

Verified customer

Submitted Nov 20 2014

Application
Western blot
Loading amount
40 µg
Gel Running Conditions
Reduced Denaturing (10% PAGE)
Sample
Human Cell lysate - whole cell (heart)
Specification
heart
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Shyam Sundar Nandi

Verified customer

Submitted Nov 19 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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