Overview

  • Product nameAnti-MYL12B antibody
    See all MYL12B primary antibodies
  • Description
    Mouse monoclonal to MYL12B
  • Tested applicationsSuitable for: ELISA, Sandwich ELISAmore details
  • Species reactivity
    Reacts with: Recombinant Fragment
    Predicted to work with: Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 73-173 of Human MYL12B (NP_2910240) with proprietary tag.

  • Positive control
    • Recombinant protein

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferpH: 7.20
    Constituent: 99% PBS
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityMonoclonal
  • IsotypeIgG1
  • Light chain typekappa

Applications

Our Abpromise guarantee covers the use of ab118096 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution.
Sandwich ELISA Use a concentration of 10 µg/ml.

Target

  • FunctionMyosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Phosphorylation triggers actin polymerization in vascular smooth muscle. Implicated in cytokinesis, receptor capping, and cell locomotion.
  • Tissue specificityUbiquitously expressed in various hematopoietic cells.
  • Sequence similaritiesContains 3 EF-hand domains.
  • Post-translational
    modifications
    Phosphorylation increases the actin-activated myosin ATPase activity and thereby regulates the contractile activity. It is required to generate the driving force in the migration of the cells but not necessary for localization of myosin-2 at the leading edge. Phosphorylation is reduced following epigallocatechin-3-O-gallate treatment.
  • Information by UniProt
  • Database links
  • Alternative names
    • ML12B_HUMAN antibody
    • MLC 2A antibody
    • MLC-2 antibody
    • MLC-2a antibody
    • MLC-B antibody
    • MLC20 antibody
    • MRLC2 antibody
    • MYL12B antibody
    • MYLC2B antibody
    • Myosin light chain 12B regulatory antibody
    • Myosin regulatory light chain 12B antibody
    • myosin regulatory light chain 2 antibody
    • Myosin regulatory light chain 2-B antibody
    • myosin regulatory light chain 2-B, smooth muscle isoform antibody
    • Myosin regulatory light chain 20 kDa antibody
    • Myosin regulatory light chain MRLC2 antibody
    • myosin, light chain 12B, regulatory antibody
    • OTTHUMP00000162244 antibody
    • OTTHUMP00000165806 antibody
    • OTTHUMP00000165807 antibody
    • OTTHUMP00000165808 antibody
    • SHUJUN-1 antibody
    • smooth muscle isoform antibody
    see all

Anti-MYL12B antibody images

  • Detection limit for recombinant GST tagged MYL12B is 0.03 ng/ml as a capture antibody. Antibody conc 10 µg/ml.

References for Anti-MYL12B antibody (ab118096)

ab118096 has not yet been referenced specifically in any publications.

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"