Overview

  • Product nameAnti-MYL7 antibody
    See all MYL7 primary antibodies
  • Description
    Mouse polyclonal to MYL7
  • Tested applicationsSuitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    MYL7 (1 a.a. ~ 175 a.a) full-length human protein.

  • Positive control
    • MYL7 transfected 293T cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab68086 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
WB 1/500 - 1/1000. Detects a band of approximately 19 kDa (predicted molecular weight: 19 kDa).

Target

  • Tissue specificityPredominantly expressed in adult atrial muscle.
  • Sequence similaritiesContains 3 EF-hand domains.
  • Information by UniProt
  • Database links
  • Alternative names
    • zgc:92755 antibody
    • atrial isoform antibody
    • cmlc2 antibody
    • MGC92755 antibody
    • MLC-2a antibody
    • MLC2a antibody
    • MLCK, cardiac antibody
    • MLRA_HUMAN antibody
    • MYL2A antibody
    • MYL7 antibody
    • Mylc2a antibody
    • Myosin light chain 2a antibody
    • Myosin light chain 7 regulatory antibody
    • Myosin light polypeptide 7 regulatory antibody
    • Myosin regulatory light chain 2 antibody
    • Myosin regulatory light chain 2 atrial isoform antibody
    • Myosin regulatory light chain 7 antibody
    see all

Anti-MYL7 antibody images

  • All lanes : Anti-MYL7 antibody (ab68086) at 1/500 dilution

    Lane 1 : MYL7 transfected 293T cell lysate
    Lane 2 : non transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 19 kDa
    Observed band size : 19 kDa
  • IF/ICC image of MYL7 antibody (ab68086) staining hES cell derived cardiomyocytes (red). The cells were fixed in paraformaldehyde and blocked in 4% goat serum for 1 hour at 25°C. The MYL7 antibody was used at a concentration of 1:100, for 16 hours at 4°C.

    See Abreview

References for Anti-MYL7 antibody (ab68086)

This product has been referenced in:
  • Ifkovits JL  et al. Inhibition of TGFß signaling increases direct conversion of fibroblasts to induced cardiomyocytes. PLoS One 9:e89678 (2014). ICC ; Mouse . Read more (PubMed: 24586958) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (hESC-derived cardiomyocyte)
Specification hESC-derived cardiomyocyte
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% Triton X-100 in PBS
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
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Submitted Nov 18 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"