The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration. Predicted molecular weight: 20 kDa.
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
FunctionMyosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Implicated in cytokinesis, receptor capping, and cell locomotion.
Tissue specificitySmooth muscle tissues and in some, but not all, nonmuscle cells.
Sequence similaritiesContains 3 EF-hand domains.
Post-translational modificationsPhosphorylation increases the actin-activated myosin ATPase activity and thereby regulates the contractile activity. It is required to generate the driving force in the migration of the cells but not necessary for localization of myosin-2 at the leading edge.
ab118896 at 10ug/ml staining MYL9 in Formalin-fixed, Paraffin-embedded Human Heart tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
References for Anti-MYL9 antibody (ab118896)
has not yet been referenced specifically in any publications.
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