ab63479 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Immunohistochemistry analysis of paraffin-embedded human brain tissue using Mylc2b (phospho S18) antibody (ab63479) at 1/50 dilution, in the presence (right panel) and absence (left panel) of immunising phosphopeptide.
Wu F et al. Identification of phosphorylated MYL12B as a potential plasma biomarker for septic acute kidney injury using a quantitative proteomic approach. Int J Clin Exp Pathol8:14409-16 (2015).
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