Overview

  • Product name
    Anti-Mylc2b (phospho S18) antibody
  • Description
    Rabbit polyclonal to Mylc2b (phospho S18)
  • Specificity
    ab63479 detects Mylc2b only when phosphorylated at serine 18 (Human: Ser18; Mouse: Ser20; Rat: Ser19).
  • Tested applications
    Suitable for: IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic phosphopeptide (Human) derived from Mylc2b from around the phosphorylation site of serine 18 (A-T-SP-N-V).

  • Positive control
    • Human brain tissue

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab63479 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab63479 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100.
ELISA 1/20000.

Target

  • Relevance
    Mylc2b (Myosin regulatory light chain 2 B, smooth muscle isoform) plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity.
  • Database links
  • Alternative names
    • Myosin light chain regulatory B antibody
    • Myosin regulatory light chain 2 B, smooth muscle isoform antibody
    • RLC B antibody

Images

  • Immunohistochemistry analysis of paraffin-embedded human brain tissue using Mylc2b (phospho S18) antibody (ab63479) at 1/50 dilution, in the presence (right panel) and absence (left panel) of immunising phosphopeptide.

References

This product has been referenced in:
  • Wu F  et al. Identification of phosphorylated MYL12B as a potential plasma biomarker for septic acute kidney injury using a quantitative proteomic approach. Int J Clin Exp Pathol 8:14409-16 (2015). Read more (PubMed: 26823757) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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