Anti-MYLIP antibody (ab107168)

Overview

  • Product nameAnti-MYLIP antibody
    See all MYLIP primary antibodies
  • Description
    Rabbit polyclonal to MYLIP
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Chicken, Cow, Dog, Pig, Chimpanzee, Zebrafish, Macaque Monkey, Gorilla
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 250 - 350 of Human MYLIP.

  • Positive control
    • This antibody gave a positive signal in the following whole cell lysates: HeLa; Jurkat; HEK293; HepG2; SHSY5Y; NIH3T3.

Properties

Applications

Our Abpromise guarantee covers the use of ab107168 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/250. Detects a band of approximately 55 kDa (predicted molecular weight: 50 kDa).

Target

  • FunctionE3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of myosin regulatory light chain (MRLC), LDLR, VLDLR and LRP8. Proteasomal degradation of MRLC leads to inhibit neurite outgrowth in presence of NGF by counteracting the stabilization of MRLC by saposin-like protein (CNPY2/MSAP) and reducing CNPY2-stimulated neurite outgrowth. Acts as a sterol-dependent inhibitor of cellular cholesterol uptake by mediating ubiquitination and subsequent degradation of LDLR.
  • Tissue specificityUbiquitously expressed.
  • PathwayProtein modification; protein ubiquitination.
  • Sequence similaritiesContains 1 FERM domain.
    Contains 1 RING-type zinc finger.
  • Developmental stageExpressed in fetal tissues and higher levels were detected in placenta and fetal lung.
  • DomainThe RING domain mediates ubiquitination and the neurite outgrowth inhibitory activity.
  • Post-translational
    modifications
    Autoubiquitinated.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • BM-023 antibody
    • BZF1 antibody
    • E3 ubiquitin protein ligase MYLIP antibody
    • E3 ubiquitin-protein ligase MYLIP antibody
    • Idol antibody
    • Inducible degrader of the LDL-receptor antibody
    • Lipoprotein receptor antibody
    • MIR antibody
    • MYLIP antibody
    • MYLIP_HUMAN antibody
    • Myosin regulatory light chain interacting protein antibody
    • PP5242 antibody
    see all

Anti-MYLIP antibody images

  • All lanes : Anti-MYLIP antibody (ab107168) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 5 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
    Lane 6 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 50 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 32 kDa,37 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 12 minutes

References for Anti-MYLIP antibody (ab107168)

ab107168 has not yet been referenced specifically in any publications.

Product Wall

Thank you for submitting an Abreview of ab107168. As you may have noticed, your review has now been published on our website.

Since you obtained poor results using the antibody in a tested species and application, we would like to follow up ...

Read More
Application Western blot
Sample Mouse Tissue lysate - whole (Left ventricle)
Loading amount 20 µg
Specification Left ventricle
Gel Running Conditions Reduced Denaturing (8%)
Blocking step Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 22°C
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Submitted Aug 29 2012

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Rat Tissue lysate - whole (Left ventricle, liver, spleen, brain)
Loading amount 20 µg
Specification Left ventricle, liver, spleen, brain
Gel Running Conditions Reduced Denaturing (8%)
Blocking step Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Aug 24 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"