Overview

  • Product nameAnti-MYLIP antibody
    See all MYLIP primary antibodies
  • Description
    Rabbit polyclonal to MYLIP
  • Specificityab74562 detects endogenous levels of total MYLIP protein.
  • Tested applicationsSuitable for: ELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    A synthesized peptide derived from an internal region of human MYLIP.

  • Positive control
    • IHC-P: human brain tissue WB: extracts from HeLa cells and Jurkat cells

Properties

Applications

Our Abpromise guarantee covers the use of ab74562 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/20000.
WB 1/500 - 1/1000. Predicted molecular weight: 50 kDa.
IHC-P 1/50 - 1/100.

Target

  • FunctionE3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of myosin regulatory light chain (MRLC), LDLR, VLDLR and LRP8. Proteasomal degradation of MRLC leads to inhibit neurite outgrowth in presence of NGF by counteracting the stabilization of MRLC by saposin-like protein (CNPY2/MSAP) and reducing CNPY2-stimulated neurite outgrowth. Acts as a sterol-dependent inhibitor of cellular cholesterol uptake by mediating ubiquitination and subsequent degradation of LDLR.
  • Tissue specificityUbiquitously expressed.
  • PathwayProtein modification; protein ubiquitination.
  • Sequence similaritiesContains 1 FERM domain.
    Contains 1 RING-type zinc finger.
  • Developmental stageExpressed in fetal tissues and higher levels were detected in placenta and fetal lung.
  • DomainThe RING domain mediates ubiquitination and the neurite outgrowth inhibitory activity.
  • Post-translational
    modifications
    Autoubiquitinated.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • BM-023 antibody
    • BZF1 antibody
    • E3 ubiquitin protein ligase MYLIP antibody
    • E3 ubiquitin-protein ligase MYLIP antibody
    • Idol antibody
    • Inducible degrader of the LDL-receptor antibody
    • Lipoprotein receptor antibody
    • MIR antibody
    • MYLIP antibody
    • MYLIP_HUMAN antibody
    • Myosin regulatory light chain interacting protein antibody
    • PP5242 antibody
    see all

Anti-MYLIP antibody images

  • All lanes : Anti-MYLIP antibody (ab74562) at 1/500 dilution

    Lane 1 : extracts from HeLa cells
    Lane 2 : extracts from Jurkat cells
    Lane 3 : extracts from Jurkat cells with immunising peptide at 10 µg

    Lysates/proteins at 10 µg per lane.


    Predicted band size : 50 kDa
    Observed band size : 50 kDa
    Additional bands at : 35 kDa. We are unsure as to the identity of these extra bands.
  • Immunohistochemistry analysis of MYLIP in paraffin embedded human brain tissue using 1/50 ab74562.
    Left image: untreated sample.
    Right image: sample treated with immunising peptide.

References for Anti-MYLIP antibody (ab74562)

This product has been referenced in:
  • Scotti E  et al. IDOL stimulates clathrin-independent endocytosis and multivesicular body-mediated lysosomal degradation of the low-density lipoprotein receptor. Mol Cell Biol 33:1503-14 (2013). WB ; Human . Read more (PubMed: 23382078) »
  • Do HT  et al. Reciprocal regulation of very low density lipoprotein receptors (VLDLRs) in neurons by brain-derived neurotrophic factor (BDNF) and Reelin: involvement of the E3 ligase Mylip/Idol. J Biol Chem 288:29613-20 (2013). WB ; Rat . Read more (PubMed: 23990472) »

See all 5 Publications for this product

Product Wall

Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (8)
Sample Human Cell lysate - whole cell (MCF-7 (breast cancer))
Specification MCF-7 (breast cancer)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Verified customer

Submitted May 21 2013

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

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Thank you for calling us and for alerting us to the problem you are experiencing with our product. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

As we discussed on the ...

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Thank you for your reply.
The CHO cells which are chinese hamster ovary cells as well as the BHK cells which are baby hamster kidney cells are untested species and I do not know if they would work with the antibody. Thus, these would not be suitab...

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Thank you for contacting us.
I am sorry to hear that this antibody is not giving you a band.
The protocol you are using seems fine to me. The WB image you sent is out of focus and MW markers are not indicated, thus it is difficult to judge if...

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Thank you for your message and for providing this further information.

I appreciate the time you have spent on these experiments, and would like to offer a free of charge replacement for this product (providing the product has been purchased i...

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Thank you for contacting us.

I am sorry to hear of the difficulties which you have been experiencing using this product. We do know that the control lysates which you have chosen should give result in western blot. Could you please tell me ab...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Heart)
Specification Heart
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Sodium Citrate Buffer
Permeabilization No
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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Submitted Sep 01 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Heart - left ventricle)
Loading amount 50 µg
Specification Heart - left ventricle
Gel Running Conditions Reduced Denaturing
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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Verified customer

Submitted Aug 30 2011

Application Western blot
Sample Rat Tissue lysate - whole (Heart - left ventricle)
Loading amount 40 µg
Specification Heart - left ventricle
Gel Running Conditions Reduced Denaturing (8)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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Verified customer

Submitted May 27 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"