Overview

  • Product nameAnti-MYLK2 antibody - N-terminal
    See all MYLK2 primary antibodies
  • Description
    Rabbit polyclonal to MYLK2 - N-terminal
  • Tested applicationsSuitable for: ELISA, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide from the N-terminal region of human MYLK2 conjugated to KLH

  • Positive control
    • Jurkat cell lysate. Human hepatocarcinoma tissue.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab71827 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1000.
IHC-P 1/50 - 1/100.
WB 1/100 - 1/500. Detects a band of approximately 55 kDa (predicted molecular weight: 65 kDa).

Target

  • FunctionImplicated in the level of global muscle contraction and cardiac function. Phosphorylates a specific serine in the N-terminus of a myosin light chain.
  • Tissue specificityHeart and skeletal muscles. Increased expression in the apical tissue compared to the interventricular septal tissue.
  • Involvement in diseaseDefects in MYLK2 are a cause of familial hypertrophic cardiomyopathy (CMH) [MIM:192600]; also designated FHC or HCM. Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.
  • Sequence similaritiesBelongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
    Contains 1 protein kinase domain.
  • Cellular localizationCytoplasm. Colocalizes with phosphorylated myosin light chain (RLCP) at filaments of the myofibrils.
  • Information by UniProt
  • Database links
  • Alternative names
    • KMLC antibody
    • MLCK antibody
    • MLCK, skeletal/cardiac antibody
    • MLCK2 antibody
    • MYLK 2 antibody
    • MYLK2 antibody
    • MYLK2_HUMAN antibody
    • Myosin light chain kinase 2 antibody
    • Myosin light chain kinase 2 skeletal muscle antibody
    • Myosin light chain kinase 2 skeletal/cardiac muscle antibody
    • MYOSIN LIGHT POLYPEPTIDE KINASE antibody
    • Myosin light polypeptide kinase, skeletal/cardiac antibody
    • Skeletal muscle myosin light chain kinase antibody
    • Skeletal myosin light chain kinase antibody
    • skeletal/cardiac muscle antibody
    • skMLCK antibody
    see all

Anti-MYLK2 antibody - N-terminal images

  • Anti-MYLK2 antibody - N-terminal (ab71827) at 1/100 dilution + Jurkat cell lysate at 12.5 µg

    Secondary
    HRP-anti-rabbit

    Predicted band size : 65 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 100 kDa,32 kDa,35 kDa,40 kDa. We are unsure as to the identity of these extra bands.
  • ab71827 at 1/50 dilution, staining MYLK2 in human hepatocarcinoma by Immunohistochemistry using formalin-fixed, paraffin-embedded tissue, followed by peroxidase-conjugated secondary antibody and AEC staining.

References for Anti-MYLK2 antibody - N-terminal (ab71827)

ab71827 has not yet been referenced specifically in any publications.

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