Overview

  • Product name
  • Description
    Rabbit polyclonal to MYLK3
  • Tested applications
    Suitable for: ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence corresponding to amino acids 27-144 of Human MYLK3. (Q32MK0).

  • Positive control
    • Human gall bladder tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab122643 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    May play a role in smooth muscle contraction.
  • Sequence similarities
    Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
    Contains 1 protein kinase domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • caMLCK antibody
    • cardiac MLCK antibody
    • Cardiac MyBP C associated Ca/CaM kinase antibody
    • Cardiac-MyBP-C-associated Ca/CaM kinase antibody
    • MLC kinase antibody
    • MLCK antibody
    • MLCK2 antibody
    • Mylk3 antibody
    • MYLK3_HUMAN antibody
    • Myosin light chain kinase 3 antibody
    • Putative myosin light chain kinase 3 antibody
    see all

Images

  • Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus but not nucleoli. Recommended concentration of ab122643 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab122643, at 1/500, staining MYLK3 in paraffin embedded Human gall bladder by Immunohistochemistry.

References

ab122643 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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