Overview

  • Product name
  • Description
    Rabbit polyclonal to MYLK3
  • Tested applications
    Suitable for: ELISA, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Human MYLK3 aa 40-69 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).

  • Positive control
    • A375 cell lysate. Human breast carcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab71826 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1000.
IHC-P 1/50 - 1/100.
WB 1/100 - 1/500. Detects a band of approximately 86 kDa (predicted molecular weight: 86 kDa).

Target

Images

  • Anti-MYLK3 antibody (ab71826) + Mouse heart lysate at 35 µg

    Predicted band size : 86 kDa
  • Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with MYLK3 antibody (N-term) (ab71826), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

  • Anti-MYLK3 antibody (ab71826) at 1/100 dilution + A375 cell lysate at 12.5 µg

    Secondary
    HRP-anti-rabbit

    Predicted band size : 86 kDa
    Observed band size : 86 kDa
    Additional bands at : 30 kDa,50 kDa,55 kDa. We are unsure as to the identity of these extra bands.
  • ab71826 at 1/50 dilution, staining Myosin light chain kinase in human breast carcinoma by Immunohistochemistry using formalin-fixed, paraffin-embedded tissue, followed by peroxidase-conjugated secondary antibody and DAB staining.

References

This product has been referenced in:
  • Miyoshi N  et al. Nodes-and-connections RNAi knockdown screening: identification of a signaling molecule network involved in fulvestrant action and breast cancer prognosis. Oncogenesis 4:e172 (2015). IHC . Read more (PubMed: 26479444) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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