- Datasheet
- Specific References
- Protocols
Overview
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Product nameAnti-MYO18A antibody
See all MYO18A primary antibodies -
DescriptionRabbit polyclonal to MYO18A
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Tested applicationsSuitable for: IPmore details
Unsuitable for: WB -
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
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Immunogen
Synthetic peptide, corresponding to a region within amino acids 300-350 of human MYO18A (NP_510880.2).
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Positive control
- RIPA whole cell lysate from HeLa cells.
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General notes
ab81689 has not performed satisfactorily when used for WB of MYO18A in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of MYO18A.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferPreservative: 0.09% Sodium Azide
Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8 -
Concentration information loading... -
PurityImmunogen affinity purified
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Purification notesab81689 was affinity purified using an epitope specific to MYO18A immobilized on solid support.
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Primary antibody notesab81689 has not performed satisfactorily when used for WB of MYO18A in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of MYO18A.
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab81689 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP | Use at 2-5 µg/mg of lysate. |
Target
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RelevanceMyo18A/Myosin XVIIIA is an unconventional myosin that contains a lysine and glutamine (KE) rich domain and a PDZ domain. The KE and PDZ domains appear to direct subcellular localization of the Myo18A variants. It is a dimeric actin binding protein that may play a role in the stabilization and organization of the actin cytoskeleton. Recently a cell surface form of Myo18A has been identified as the receptor for surfactant protein A, a lipid associated lung collectin that mediates innate and adaptive immune responses in the lungs.
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Cellular localizationIsoform 1: Endoplasmic reticulum Golgi intermediate compartment. Cytoplasm, cytoskeleton. Note: Colocalizes with actin. Isoform 2: Cytoplasm. Note: Lacks the PDZ domain. Diffusely localized in the cytoplasm.
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Database links
- Entrez Gene: 399687 Human
- Omim: 610067 Human
- SwissProt: Q92614 Human
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Alternative names
- MAJN antibody
- Molecule associated with JAK3 N terminus antibody
- Myosin 18A antibody
see all
Anti-MYO18A antibody images
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Immunoprecipitation - MYO18A antibody (ab81689)Detection of human MYO18A by Western Blot of Immunoprecipitates.
Samples: RIPA whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells.
ab81689 used for IP at 3 µg/mg lysate (lane 1).
MYO18A was also immunoprecipitated using anti MYO18A (ab81690), which recognizes a downstream epitope (lane 2).
Lane 3 represents the control IgG.
For blotting immunoprecipitates, ab81689 was used at 1 µg/ml.
Detection: by Chemiluminescence with an exposure time of 1 second.
Protocols
References for Anti-MYO18A antibody (ab81689)
ab81689 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"