Overview

  • Product name
    Anti-MYO18A antibody
    See all MYO18A primary antibodies
  • Description
    Rabbit polyclonal to MYO18A
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IPmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 300-350 of human MYO18A (NP_510880.2).

  • Positive control
    • RIPA whole cell lysate from HeLa cells.
  • General notes


    ab81689 has not performed satisfactorily when used for WB of MYO18A in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of MYO18A.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab81689 was affinity purified using an epitope specific to MYO18A immobilized on solid support.
  • Primary antibody notes
    ab81689 has not performed satisfactorily when used for WB of MYO18A in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of MYO18A.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab81689 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-5 µg/mg of lysate.
  • Application notes
    Is unsuitable for WB.

    • Target

    • Relevance
      Myo18A/Myosin XVIIIA is an unconventional myosin that contains a lysine and glutamine (KE) rich domain and a PDZ domain. The KE and PDZ domains appear to direct subcellular localization of the Myo18A variants. It is a dimeric actin binding protein that may play a role in the stabilization and organization of the actin cytoskeleton. Recently a cell surface form of Myo18A has been identified as the receptor for surfactant protein A, a lipid associated lung collectin that mediates innate and adaptive immune responses in the lungs.
    • Cellular localization
      Isoform 1: Endoplasmic reticulum Golgi intermediate compartment. Cytoplasm, cytoskeleton. Note: Colocalizes with actin. Isoform 2: Cytoplasm. Note: Lacks the PDZ domain. Diffusely localized in the cytoplasm.
    • Database links
      • Alternative names
        • MAJN antibody
        • Molecule associated with JAK3 N terminus antibody
        • Myosin 18A antibody
        • Myosin containing a PDZ domain antibody
        • Myosin XVIIIA antibody
        • MyoXVIIIIA antibody
        • MYSPDZ antibody
        • SPR210 antibody
        see all

      Images

      • Immunoprecipitation - Anti-MYO18A antibody (ab81689)
        Immunoprecipitation - Anti-MYO18A antibody (ab81689)
        Detection of human MYO18A by Western Blot of Immunoprecipitates.
        Samples: RIPA whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells.
        ab81689 used for IP at 3 µg/mg lysate (lane 1).
        MYO18A was also immunoprecipitated using anti MYO18A (ab81690), which recognizes a downstream epitope (lane 2).
        Lane 3 represents the control IgG.
        For blotting immunoprecipitates, ab81689 was used at 1 µg/ml.
        Detection: by Chemiluminescence with an exposure time of 1 second.

      References

      ab81689 has not yet been referenced specifically in any publications.

      Publishing research using ab81689? Please let us know so that we can cite the reference in this datasheet.

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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