Overview

  • Product name
  • Description
    Rabbit polyclonal to MYO18A
  • Tested applications
    Suitable for: IPmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rat, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide, corresponding to a region between residue 2004-2054 of human MYO18A (NP_510880.2).

  • Positive control
    • RIPA whole lysates from HeLa cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab81690 was affinity purified using an epitope specific to MYO18A immobilized on solid support.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab81690 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP
  • Application notes
    IP: Use at 2-5 µg/mg of lysate.

    Is unsuitable for WB.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance
      Myo18A/Myosin XVIIIA is an unconventional myosin that contains a lysine and glutamine (KE) rich domain and a PDZ domain. The KE and PDZ domains appear to direct subcellular localization of the Myo18A variants. It is a dimeric actin binding protein that may play a role in the stabilization and organization of the actin cytoskeleton. Recently a cell surface form of Myo18A has been identified as the receptor for surfactant protein A, a lipid associated lung collectin that mediates innate and adaptive immune responses in the lungs.
    • Cellular localization
      Isoform 1: Endoplasmic reticulum Golgi intermediate compartment. Cytoplasm, cytoskeleton. Note: Colocalizes with actin. Isoform 2: Cytoplasm. Note: Lacks the PDZ domain. Diffusely localized in the cytoplasm.
    • Database links
    • Alternative names
      • MAJN antibody
      • Molecule associated with JAK3 N terminus antibody
      • Myosin 18A antibody
      • Myosin containing a PDZ domain antibody
      • Myosin XVIIIA antibody
      • MyoXVIIIIA antibody
      • MYSPDZ antibody
      • SPR210 antibody
      see all

    Images

    • Detection of human MYO18A by Western Blot of Immunoprecipitates.
      Samples: RIPA whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells.
      ab81690 used for IP at 3 µg/mg lysate (lane 2).
      MYO18A was also immunoprecipitated by rabbit anti MYO18A (ab81689), which recognizes an upstream epitope (lane 1).
      Lane 3 represents the control IgG.
      For blotting immunoprecipitates, ab81689 was used at 1 µg/ml.
      Detection: by Chemiluminescence with an exposure time of 1 second.

    References

    ab81690 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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